Examination of Bioartifichial Pancreac for future clinical use.

生物人工胰腺被设计成结合胰岛组织和选择性渗透膜，将胰岛与受体的免疫系统隔离开来。本研究的目的是研究含有异体胰岛和异体胰岛的琼脂糖微胶囊在犬模型上作为生物人工胰腺的可能性。同种异体移植：采用5只完全胰腺切除的糖尿病犬作为受体，无免疫抑制，测定微囊化胰岛的功能效果。通过导管将一定数量的异交杂种供体的微囊化胰岛移植到切除胰腺的受体大网膜组织中。5名接受胰岛素治疗的患者中，有3人在不使用外源性胰岛素的情况下，连续28、42、49天将平均空腹血糖控制在120 mg/dl以下，分别接受4.3*103、7.3*103和1.0*104 （IE/kg）微囊化胰岛。移植：我们已经证明琼脂糖-聚苯乙烯磺酸混合物制成的微胶囊有效地延长了啮齿动物模型的异种胰岛存活时间。我们供应琼脂糖和聚乙烯磺酸给奈良医学院的研究小组。他们将我们的微胶囊应用于猪胰岛移植给犬受体。正常血糖期分别为14、42、89、99天。总之，本研究表明，微囊化胰岛可以在大型糖尿病动物中发挥作用，从而在不需要免疫抑制的情况下长时间不依赖外源性胰岛素治疗。

The bioartificial pancreas was designed to incorporate islet tissues and a selectively permeable membrane that isolates islets from the immune system of the recipient. The aim of this study is to examine the possibility of the agarose microcapsule containing allo-islets and xeno-islet as a bioartificial pancreas in canine model.Allograft : Functional efficacy of the microencapsulated islets was determined using five totally-pancreatectomized diabetic dogs as recipients without immunosuppression. Defined quantity of microencapsulated islets from outbred mongrel donors were grafted through the catheter into omental tissue of the pancreatectomized recipients. In three of five recipients, the average fasting glucose values were controlled under 120 mg/dl for 28,42,49 days without exogenous insulin, which received totally 4.3*103,7.3*103 and 1.0*104 (IE/kg) of microencapsulated islets, respectively.Xnoe-graft : We have demonstrated that the microcapsule made of an agarose-polystirene sulfonic acid mixture effectively prolonged xeno-islet survival period in rodent models. We supply the agarose and polystirene sulfonic acid to the research group of Nara medical school. They applied our microcapsule to transplantation of porcine islet to canine recipients. The normoglycemic periods of the recipients are 14,42,89, and 99 days.In conclusion, the present study indicates that the microencapsulated islets can function in large diabetic animals, resulting in the independence of exogenous insulin therapy for prolonged periods without the need for immunosuppression.