Molecular epidemiology of hepatitis G virus infection

我们最近在东南亚国家发现了一种新的庚型肝炎病毒（HGV）基因型。为了更详细地描述病毒基因组，我们克隆了属于新基因型的两种不同HGV分离株的全长核苷酸序列。这些分离株分别从缅甸人（命名为HGV-MY 14）和越南人（命名为HGV-VT 48）受试者的血清中回收。两株HGV均由9228个核苷酸（nt）组成，开放阅读框为8529个核苷酸，编码2843个氨基酸残基。该分离株与先前报道的HGV/GBV-C分离株（由1型至3型组成）有13-15%的核苷酸序列和4-6%的氨基酸序列不同。这两个分离物缺乏一个明确的可识别的核心基因，只有16个氨基酸残基。基于全基因组序列的系统发育分析，HGV-MY 14和VT 48分离物可以被归类为新的基因型以及5 '-UTR序列的结果，并将该基因型命名为4型。

We recently identified a novel genotype of hepatitis G virus (HGV) in Southeast Asian countries. To characterize the viral genome in more detail, we cloned the full-length nucleotide sequence of the two different HGV isolates belonging to the novel genotype. These isolates were recovered from serum of Myanmarese (designated HGV-MY14) and Vietnamese (designated HGV-VT48) subjects, respectively. Both HGV isolates were composed of 9228 nucleotides (nt) and had a single open reading frame spanning 8529 nt and encoding 2843 amino acid (aa) residues. This isolate differed from previously reported HGV/GBV-C isolates consisting of type 1 through 3, by 13-15% of the nucleotide sequence and 4-6% of the amino acid sequence. Both isolates lacked a clearly identifiable core gene that had only 16aa residues. Based on phylogenetic analysis of the full-genome sequence, HGV-MY14 and VT48 isolates can be classified into the novel genotype as well as the result in the 5'-UTR sequence, and named this genotype, type 4.