Neuronal connection of the nociceptive and pain-modulating systems, with special reference to peptides and their receptors involved in the systems.

1.结果：1.向导水管周围灰质发出轴突的NK 1免疫阳性神经元主要分布于大鼠延髓和脊髓背角的I层、脊髓外侧核，此外还分布于脊髓的V层和X层.研究了NK 1在新生儿和成人脊髓中的分布.在大鼠延髓和脊髓背角I、II层的P物质或CGRP免疫反应阳性轴突终末中发现μ阿片受体免疫反应阳性.在大鼠延髓背角的部分三叉神经丘脑神经元中发现了前强啡肽原免疫反应阳性细胞.在细胞内记录和标记实验中，在大鼠延髓的切片制备，局部回路神经元在第二层的延髓背角被分为两种类型的基础上，他们的电膜特性和轴突分支模式。一种类型的局部回路神经元群体显示钙结合蛋白D28 K免疫反应性。免疫反应性钙结合蛋白（小白蛋白，calretinin，calbindin D28 K）在延髓背角神经元，发送其轴突的臂旁，丘脑，或下丘脑区域的大鼠。当10%福尔马林皮下注射到口周区域时，这些神经元中的一些被证实表达c-Fos免疫反应性。

1. NK1-immunoreactive neurons sending their axons to the periaqueductal gray were distributed mainly in lamina I of the medullary and spinal dorsal horns, spinal lateral nucleus, and additionally in laminae V and X of the spinal cord in the rat.2. Distribution of NK1-immunoreactivity was examined in the newborn and adult human spinal cords.3. Mu-opioid receptor-immunoreactivity was found in substance P- or CGRP-immunoreactive axon terminals in laminae I and II of the medullary and spinal dorsal horn of the rat.4. Preprodynorphin-immunoreactivity was found in some trigeminothalamic neurons in the medullary dorsal horn of the rat.5. In intracellular recording and labeling experiments in slice preparations of the rat medulla oblongata, local circuit neurons in lamina II of the medullary dorsal horn were divided into two types on the basis of their electrical membrane properties and axonal arborization patterns. A population of the local circuit neurons of one type showed calbindin D28K-immunoreactivity.6. Immunoreactivities for calcium-binding proteins (parvalbumin, calretinin, calbindin D28K) were observed in medullary dorsal horn neurons which send their axons to the parabrachial, thalamic, or hypothalamic regions in the rat. Some of these neurons were confirmed to express c-Fos-immunoreactivity when 10% formalin was injected subcutaneously into the perioral regions.