Functional significance of inhibitory interneurons in the neuronal mechanisms of mastication - the intracellular recording and labeling and immuno-gold labeling techniques.

据报道，在三叉神经运动神经元上突触的rostrodormedidial oral （Vo.r）神经元使用抑制性神经递质。因此，首先将Nb和HRP注射成Vo。在猫的合颌运动核（JC）和开颌运动核（JO）中分别选取r神经元和α-运动神经元，以确定它们之间接触的数量和空间分布。R神经元和运动神经元。单一的签证官。r个神经元终止于JC核(Vo。r-dl神经元)发出的钮扣平均是Vo的10倍。r神经元终止于JO核(Vo。r-vm神经元)。签证官。r-dl神经元- jc α-神经神经元对和Vo。r-vm神经元- jo运动神经元对在运动神经元的胞体或树突上平均分别进行5次和4次接触。这些结果表明，Vo的整体抑制作用。JC内的r神经元数量远大于JO。确定接受抑制接触的JC α-运动神经元和JO α-运动神经元之间树突树的几何相似性和差异性也非常重要。因此，在细胞内用HRP标记JC和JO α-运动神经元，并使用计算机辅助三维系统进行定量分析。JC α-运动神经元的树突树与细胞体的位置无关，均局限于JC运动核内。而JO α-运动神经元在网状结构中产生广泛的核外树突。JC近端树突节段的分支模式比JO α-运动神经元更简单。尽管存在这些差异，但两种α-运动神经元每个神经元的树突参数平均值并无差异，且茎突直径与多个树突参数呈线性正相关。这些结果表明，树突状结构的基本设计原则与支配拮抗肌肉的三叉神经α-运动神经元树突状结构和分支模式的差异无关。少

Is has been reported that rostrodorsomedial oralis (Vo.r) neurons synapsing on trigeminal motoneurons use inhibitory neurotransmitters. At first, therefore, injections of Nb and HRP were made into a Vo.r neuron and an α-motoneuron in the jaw-closing (JC) and jaw-opening (JO) motor nucleus, respectively, in cats in order to determine the number and spatial distribution of contacts between the Vo.r neuron and motoneuron. Single Vo.r neurons terminating in the JC nucleus (Vo.r-dl neurons) issued on average 10 times more boutons than Vo.r neurons terminating in the JO nucleus (Vo.r-vm neurons). The Vo.r-dl neuron-JC α-mosoneuron pairs and the Vo.r-vm neuron-JO motoneuron pairs made on average 5 and 4 contacts, respectively, on the soma or dendrites of the motoneurons. These results suggest that the overall inhibitory effect exerted by Vo.r neurons is much greater within JC than JO motoneuron pool.It is also very important to determine geometrical similarities and differences of the dendrit … More ic tree between the JC and JO α-motoneurons, which receive the inhibitory contacts. Thus, JC and JO α-motoneurons were intracellulary labeled with HRP and quantitatively analyzed using a computer-assisted three-dimensional system. The dendritic tree of JC α-motoneurons was confined within the JC motor nucleus, despite locations of the cell body. Whereas, JO α-motoneurons generated extensive extranuclear dendrites in the reticular formation. The branching pattern of proximal dendritic segments was simpler in the JC than JO α-motoneurons. Despite these differences, the mean values of dendritic parameters examined per neuron were not different between the two kinds of α-motoneurons, and the stem dendrite diameter was positively correlated with several dendritic parameters in a linear manner. These results suggest that underlying design principles of the geometry of dendritic tree are not concerned with the differences in configuration and branching pattern of the dendritic tree of trigeminal α-motoneurons innervating antagonistic muscles. Less