Creation of high-quality COaAp-collagen composites for reconstruction and regeneration of alveolar bone

近年来，支架生物材料成为组织工程领域的研究热点。将含镁功能梯度碳磷灰石与胶原蛋白混合，制得FGMgCO3AP-胶原复合材料。对磷灰石晶体进行梯度的镁离子修饰，促进了细胞的粘附性。此外，作为支架材料，FGMgCO_3AP-胶原复合材料可促进骨形成。镁离子可能参与破骨细胞的骨代谢和成骨细胞与其细胞表面整合素的作用。此外，为了开发一种新型的可生物降解支架生物材料，将合成的CO3AP与中和的胶原凝胶混合，并将其冷冻干燥成海绵，形成带孔的HAP框架环。纯胶原海绵样品上的基质物质随着培养时间的延长而增加，海绵表面几乎被覆盖。苏木精-伊红染色切片证实，成骨细胞已很好地侵入了CO_3AP-胶原海绵。当将这些海绵-框架复合体植入大鼠骨膜骨膜下时，从颅骨膜表面向复合体的内核方向观察到新生骨。这些增强的CO3AP-胶原海绵有望作为治疗用的硬组织支架生物材料。

Recently, scaffold biomaterials have been focused in the tissue engineering field. FGMgCO_3Ap-collagen composite was made by mixing the functionally graded CO_3apatite containing Mg with collagen. Modification of gradational Mg^<2+> ions on the apatite crystals promoted cell adhesion. In addition, as a scaffold material, the FGMgCO_3Ap-collagen composite suggested contributing to bone formation. Mg^<2+> ions may contribute to the bone metabolism of osteoclast and osteoblast action with the integrins at their cell surfaces.Furthermore, to develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized CO_3Ap was mixed with a neutralized collagen gel and the CO_3Ap-collagen mixtures were lyophilized into sponges in a HAp frame ring with pores. Matrix substances on the pure collagen sponge samples increased with the culture period and the surface of the sponge was almost covered. A sectional view of hematoxylin-eosin staining confirmed that osteoblast cells had well invaded the CO_3Ap-collagen sponge. When these sponge-frame complexes were implanted beneath the periosteum crane of rats, newly created bone was observed toward the inner core of the complex from the surface of the periosteum cranii. These reinforced CO_3Ap-collagen sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic purposes.