The Dietary Factor of Insulin Resistance ; Adiponectin and Saturated Fatty Acid

胰岛素抵抗是代谢综合征丛集性疾病病因学的核心。流行病学和临床证据表明，饮食中摄入大量脂肪与胰岛素抵抗有关。近年来，胰岛素抵抗与细胞因子的相关性有报道。本研究的目的是阐明脂肪摄入量或脂肪酸组成对餐后血糖水平、血清胰岛素反应和脂肪细胞因子的影响。首先，10名健康的年轻女性食用两种低脂肪（F-20:20%总能量）等能研究饮食；高脂肪（f -30:30%的能量），两者的能量和蛋白质含量相似（占总能量的15%），在不同的场合，每隔3周持续5天。在最后一天进行膳食耐受性试验，因此在试验餐前、30分钟、60分钟和120分钟后进行血样采集。F-20和F-30之间的HOMA-R没有区别。TNF-α在F-3和F-20中的表达明显高于F-20。TNF-α与CRP呈显著正相关，TNF-α与体脂量呈显著正相关。其次，10只健康的年轻雌性分别食用两种等能饮食，一种是高脂肪（f -30:30%占总能量，S:M:P=3:4:3），另一种是高饱和脂肪酸（f -30:30%脂肪占总能量，S:M:P=5:4:1），这两种饮食的能量和蛋白质含量相似（占总能量的15%），持续5天，时间间隔至少为3周。HOMA-R在F-30和FB-30之间没有变化。FB-30组LDL-C明显高于F-30组。试验餐后120分钟，FB-30组的甘油三酯和RLP-C水平显著高于F-30组。此外，F-30和FB-30日粮中RLP-C与脂联素水平均呈显著正相关。TNF-α在FB-30中显著高于F-30， TNF-α (FB-30/F-30)的变化与HOMA-R水平（FB-30/F-30）的变化呈正相关。这些结果提示饱和脂肪酸可能通过增加TNF-α水平在胰岛素抵抗的发展中起关键作用。少

Insulin resistance is central for the etiology of the metabolic syndrome cluster of disease. Epidemiological and clinical evidence suggests that a high intake of dietary fat is associated with insulin resistance. Recently, the correlation of the insulin resistance and cytokine has been reported. The aims of this study were to clarify the effects of either amount of fat intakes or fatty acid composition on the postprandial plasma glucose level, the serum insulin response, and adipocytokine. Preliminary, ten healthy young females were consumed two isoenergestic study diets with a low fat (F-20:20% of total energy); a high fat (F-30:30%of energy), both of which had similar energy and protein contents (15% of total energy) for 5days, on separate occasions, at intervals of 3 weeks. On the last day, the meal tolerance test was consumed, therefore, blood sampling was done before and 30, 60, and 120 minutes after the test meals. The HOMA-R did not differ between F-20 and F-30. The TNF-α in F-3 … More 0 was significantly higher than that of F-20. A statistically significant positive correlations were observed between the TNF-α and the CRP, and the TNF-α and the body fat mass, respectively. Secondary, ten healthy young females were consumed two isoenergestic study diets with high fat (F-30:30%of total energy, S:M:P=3:4:3), a high saturated fatty acid (FB-30:30% fat of total energy, S:M:P=5:4:1), which had similar energy and protein contents (15% of total energy) for 5days, on separate occasions, at intervals of least 3 weeks. The HOMA-R did not change between F-30 and FB-30. The LDL-C in FB-30 was significantly higher than that of F-30. The triglyceride and the RLP-C levels at 120minutes after test meals were significantly greater in FB-30 compared to F-30. In addition, the significantly positive correlations were observed between the RLP-C and adiponectin level in both of F-30 and FB-30 diet. The TNF-α in FB-30 was significantly greater than in F-30, and there were positive correlation between the changes of TNF-α (FB-30/F-30)and that of HOMA-R level (FB-30/F-30). These results suggest that saturated fatty acid may play a key role in the development of insulin resistance due to increase the TNF-α level. Less