Development of Dual-Tandem-Mass Spectrometer with a New Ion-trap and Its Application to Analysis of Molecular Nano-dynamics of a Cell

1.新型离子阱双串联质谱仪的研制对一种新的三极概念进行了计算机模拟，发现这种新的三极不仅可以用于滤质器，而且可以用于由两个三极组成的新型间隙离子阱。利用这些新的离子光学器件，我们设计了一种新型的双串联质谱仪，它包括两个滤质器、一个碰撞池和一个飞行时间质谱仪，可以对分子进行定量和定性分析。研制了试验机，并对其性能进行了检验.活单细胞质谱分析有了这种灵敏度高、功能多的新型质谱仪，我们可以将这种类型的质谱仪应用于活单细胞分析。然而，单个细胞的分子量非常小，每个分子种类有一百万个分子。因此，它以前没有成功过。我们最终发现了一种新的超灵敏电离方法，即在视频显微镜下将纳米电喷雾尖端插入单个细胞中，吸取细胞内容物，并将尖端直接用作纳米电喷雾电离源。该方法成功地引入了细胞的分子组成，检测了数百个分子峰，将应用于生命科学的各个方面，加快了生命科学分析的速度。

1. Development of Dual-Tandem-Mass Spectrometer with New Ion-trapA new concept of tripole was simulated by computer and it was found that this new tripole can be used not only for mass filter but also for a new gap ion trap which consists of two tripoles. With these new ion optics, a new dual tandem mass spectrometer which has two mass filter, one collision cell and one time-of-flight mass spectrometer, by which we can analyze molecules in quantitative and qualitative ways. The test machine was developed and its performance was checked.2. Live Single Cell Mass Spectrometric AnalysisWith such sensitive and versatile new mass spectrometer, we can apply this type of mass spectrometer for live single cell analysis. However, the molecular amount of a single cell is very tiny with a million molecules for each molecular species. Thus it has not been succeeded before. We finally found a new super sensitive ionization methodology in which we inserted a nano-electro spray tip into a single cell under video-microscope and suck the contents of a cell and the tip was directly used as a nano-electro spray ionization source. This method is so successful to introduce the molecular contents of a cell and to detect hundreds of molecular peaks.This new method will be used in every aspect of bio-sciences and will accelerate the speed of analyses of life sciences.