Linking mutant zebrafish phenotypes with their underlying genetic lesions.

斑马鱼突变体已经产生了许多有趣的异常，但为了理解这些异常，缺陷基因必须通过定位克隆来识别。我们试图确定支撑四个突变体的缺陷基因。突变体#562发育出一个正常的神经系统，然后经历快速退化。突变的Flotte Lotte的肠道发育异常。将对两个早期造血缺陷的突变体进行研究。我们将建立几个步骤的技术，这些技术将对所有斑马鱼测绘项目有用。我们希望这些突变体的基因特征能够为神经细胞的存活、肠道发育和血液形成提供新的见解。

Zebrafish mutants have been generated with many interesting abnormalities, but to understand these abnormalities, the defective genes must be identified by positional cloning. We seek to identify the defective genes underpinning four mutants. Mutant #562 develops a normal nervous system which then undergoes rapid degeneration. The mutant flotte lotte has abnormal gut development. Two mutants with defective early blood formation will be studied. We will establish techniques for several steps that will be useful for all zebrafish mapping projects. We expect the genetic characterization of these mutants to provide new insights into nerve cell survival, gut development, and blood formation.