Diagnosis of Oral Intraepithelial Neoplasia and Study for Mucosal Tissue Stem Cells Using Our Original Tissue Q-FISH Method.

我们可以用我们独创的组织Q-FISH方法测量载玻片上每个细胞群的端粒长度。通过对口腔黏膜组织的分析，我们可以证实，在上皮细胞中，基底细胞的端粒最长，这表明组织干细胞存在于基底层。我们也可以证明一些不典型的病变作为原位癌，因为有短端粒的粘膜癌相同的模式。癌旁上皮细胞或某些白斑细胞端粒明显缩短，染色体不稳定，可能是真正的癌前病变。

We can measure the teIomere length in each cell group on the slide by using our original tissue Q-FISH method. In analysis of oral mucosa, we could confirm that the basal cells have the longest telomeres among the epithelial layer, and that suggests the tissue stem cells are reside in the basal layer. We could also have proved some dysplastic lesions as kinds of carcinoma in situ, because of having short telomeres as same pattern as mucosal carcinomas. The epithelia adjacent to carcinomas or some leukoplakias have significantly short telomeres and chromosomal instabilities, therefore, these lesions might be the true precancerous lesions.