Study on molecular mechanism of oxidative stress in brain and anti-oxidant interventions

我们使用一种新的光子成像方法“实时生物放射成像”检测了体外脑片中依赖于活性氧自由基的化学发光信号。结果如下：(1)缺氧-复氧和低氧分别促进了活性氧自由基和葡萄糖代谢的产生。(2)线粒体被认为是活性氧自由基产生的主要部位。(3)为了研究减缓氧化应激的干预措施，我们研究了限制热量对活性氧自由基产生的影响。然而，限制卡路里并没有改变活性氧自由基的产生。

We examined reactive oxygen radical-dependent chemiluminescent signals in ex-vivo brain slices using a novel photonic imaging method "real-time bioradiography". Results are as follows.(1) Reactive oxygen radical generation and glucose metabolism was enhanced during hypoxia-reoxygenation and hypoxia, respectively.(2) Mitochondria are suggested as major sites of reactive oxygen radical generation.(3) To develop the interventions to decelerate oxidative stress, we examined effect of calorie restriction on reactive oxygen radical generation. However, calorie restriction did not change the reactive oxygen radical generation.