Molecular and morphological analysis of RNA helicase that shows an unique expression in the nervous system by utilizing genetically modified animals

制备了特异性识别少突胶质细胞的单克隆抗体（4F2），该抗体识别的分子被证明是RNA解旋酶的成员Ddx 54。在强制表达Ddx 54的转基因动物中，髓鞘形成似乎加速。4F2阳性细胞在大鼠中枢神经系统从早期胚胎到成年阶段观察到，它们特异性地表达在少突胶质细胞中使用神经组织的原代培养物。Ddx 54与4种MBP亚型结合，尤其是21。MBP的5kDa亚型被转运到细胞核中，表明其参与了少突胶质细胞髓鞘形成过程中的信号转导。

The monoclonal antibody(4F2) that specifically identifies oligodendrocytes was generated, and the molecule recognized by this antibody proved to be Ddx54, a member of RNA helicase. In the transgenic animals with forced expression of Ddx54, myelination appeared to be accelerated. The 4F2-positive cells were observed in the rat central nervous system from early embryonal to adult stages, and they were specifically expressed in oligodendrocytes using primary cultures of neural tissues. Ddx54 bound to 4 types of MBP isoforms, and especially the 21. 5 kDa isoform of MBP was transported into the nucleus, suggesting its involvement in the signal transduction during the process of myelination in oligodendrocytes.