Research on early diagnosis of abnormal carbohydrate metabolism by antibody specific for AGEs

戊苷是一种晚期糖基化终产物（AGEs），通过高效液相色谱法测定血清中的戊苷，并通过温度和时间依赖性的加热方式增加戊苷的含量。戊苷形成的增加受到酸性条件的显著抑制。说明在加热过程中加入盐酸可使酶联免疫吸附法测定血清戊苷水平更加准确。此外，N^ω-羧甲基精氨酸（CMA）是一种酸不稳定的AGE结构，在糖基化胶原蛋白中优先生成。我们阐明了糖化胶原蛋白中CMA形成的热点。

Pentosidine, one of advanced glycation end-products(AGEs), is measured by HPLC in the serum, and increased by heating in a temperature-and time-dependent manner. The increase in pentosidine formation is significantly inhibited by acidic conditions. This indicates that the level of serum pentosidine will be measured more accurately by ELISA if hydrochloric acid is added during the heating process. Furthermore, N^ω-carboxymethylarginine(CMA), acid labile AGE structure, is preferentially generated in glycated collagen. We elucidated the hot-spot of CMA formation in glycated collagen.