The efficacy of anti-Aβoligomer antibody to the toxicity of Aβto neuronal cells

最常见的痴呆类型是阿尔茨海默病（AD）。AD的病因被认为是由淀粉样前体蛋白（APP）裂解而成的淀粉样β蛋白（Aβ）具有神经毒性，可诱导神经细胞死亡。近年来有研究发现可溶性寡聚体对突触有毒性作用，可导致认知功能下降。我们认为抗寡聚体抗体具有神经保护作用。本研究探讨了抗寡聚体抗体对Aβ毒性神经细胞的保护作用机制。我们研究了当Aβ自身聚集时，抗寡聚体抗体是否减少自由基形成的量。与对照溶液相比，自由基形成量减少。在培养液中加入抗寡聚体抗体和Aβ后，PC12细胞的存活率明显提高。结果发现，在含有抗寡聚体抗体的培养液中加入Aβ后，PC12细胞NMDA受体的数量发生了变化。我们没有证实抗寡聚体抗体对NMDA受体的作用是直接的还是间接的。我们认为这两个性质之间存在着某种联系。

The most popular type of dementia is Alzheimer's disease(AD). The cause of AD is thought that amyloid beta protein(Aβ) cleaved from amyloid precursor protein(APP) which is expressing on the neuronal cell membranes has neurotoxicity and induce neuronal cell death. Recently some researchers found the soluble oligomer form had toxicity to the synapses and induce cognitive decline. We thought that anti-oligomer antibody has neuroprotective effect. We investigated the mechanisms of neuroprotective effect of anti-oligomer antibody against neuronal cells from Aβtoxicity. We investigated whether or not anti-oligomer antibody reduce the amount of free radical formation when Aβaggregate themselves. The amount of free radical formation was reduced compared with control solution. The viability of PC12 cells was higher when culture medium was added anti-oligomer antibody with Aβ. We found some changes of amount of NMDA receptor in PC12 when added Aβto culture medium with anti-oligomer antibody. We did not confirm whether the effect of anti-oligomer antibody against NMDA receptor was direct or indirect effect. We thought that there are some relations between two properties.