Investigation of role of glial cells in glaucomatous optic neuropathy and searching glial cell-related treatment for glaucoma

阐明了以下几点：不同的谷氨酸转运体（GLT）参与了胶质细胞对RGC的影响。主要GLT、GLAST对RGC有神经保护作用，而GLT-1有促进神经炎的作用。视网膜主要的胶质细胞Muller细胞对视网膜神经节细胞（RGCs）有保护作用，而视神经中的星形胶质细胞对神经突起有促进作用。几种影响RGC存活或轴突伸长的mRNA被鉴定为GLT参与RGC效应的关键mRNA。EGFR、Gabbr 2、Agtr 1a对RGC的死亡有保护作用，Id 3、Egr 1、Gja 1 p、App、Nr 2f 2 mRNA参与RGC的死亡。我开发了一种动物模型，能够观察活体大鼠视网膜的轴突流动。

I clarified the following points ; Different glutamate transporters(GLT) were differently involved in glial cell-related effect on RGC. Major GLT, GLAST exerted neuroprotective effect on RGC, while GLT-1 exerted effect on promoting neuritis. Muller cells, major retinal glial cells, showed a protective effect on retinal ganglion cells(RGCs), while astrocytes in optic nerve did promoting effect of neurites. Several mRNAs influenced to RGC survival or neurite elongation were identified as key mRNAs for GLT-involved RGC effects. EGFR, Gabbr2, Agtr1a showed protective effect, while Id3、Egr1, Gja1p, App, Nr2f2 mRNAs were involved in RGC death. I developed an animal model that enables to observe axonal flow of lived rat retina.