Genes associated with brain-tropic human melanoma cells and their function

转移形成是大多数癌症死亡的原因。脑转移，最常见于黑色素瘤，如肺癌和乳腺癌，引起高发病率和死亡率。我们使用生物发光成像（BLI）技术分析了许多人类癌细胞系，证明了NOD/SCID小鼠的癌细胞类型依赖转移到特定器官。本研究在小鼠心脏内连续接种3个周期后，建立了嗜脑性黑色素瘤细胞系SK-MEL-28-luc-br2和Mewo-luc-br2，并测定了注射后黑色素瘤细胞在脑内蓄积的动力学。注射后0.5-5小时，脑致性和低转移性黑色素瘤（SK-MEL-2-luc）细胞在脑内的存在程度相似，但脑致性黑色素瘤细胞在晚期（15-20天后）肿瘤源光子显著增加。进一步的综合转录组分析显示，tgf - β受体II在嗜脑黑色素瘤和乳腺癌（MDA-MB-231-luc-brl）细胞中显著增加（3-6倍）。bli介导的体内成像应该促进潜在的肿瘤转移分子标志物的识别。

Metastasis formation is responsible for most cancer deaths. Brain metastases, most often with melanomas as with lung and breast carcinomas, induce a high morbidity and mortality. Our analysis of many human cancer cell lines using bio-luminescent imaging (BLI) technology, has demonstrated cancer cell-type dependent metastasis to specific organs of NOD/SCID mice. Herein we established brain-tropic melanoma cell lines (SK-MEL-28-luc-br2 and Mewo-luc-br2) after 3 cycles of sequential intra-cardiac (i.e.) inoculation into mice, and determined the kinetics of brain accumulation of melanoma cells after i.e. injection. While both the brain-tropic and the low-metastatic melanoma (SK-MEL-2-luc) cells were present to similar degrees in the brain 0.5-5 hrs after injection, brain-tropic melanoma cells showed a significant increase in tumor-derived photons at the late phase (after 15-20 days). Further a comprehensive transcriptome analysis showed a significant increase (3-6 fold) of TGF-beta receptor II in brain-tropic melanoma and breast carcinoma (MDA-MB-231-luc-brl) cells. The BLI-mediated in vivo imaging should promote identification of potential molecular markers for cancer metastasis.