Histochemical studies on the expression patterns and dynamical changes of carbohydrates

50多种凝集素和糖特异性单克隆抗体应用于各种人和小鼠组织标本的组织化学。在实质细胞和血管内皮细胞上都证明了糖部分的器官特异性分布。内皮细胞表面糖的特异性分布可能是解释肿瘤器官特异性转移机制的重要线索。在包括糖尿病在内的各种病理状态下，糖脂在大鼠大脑、小肠和肾脏中也有特异性分布。组织化学方法揭示了糖表达在各种组织和细胞上的动态变化的重要方面。

More than fifty kinds of lectins and sugar-specific monoclonal antibodies were histochemically applied to various kinds of human and mouse tissue specimens. Organ-specific distribution of sugar moieties were demonstrated on both parenchymal cells and vascular endothelial cells. Specific distribution of endothelial cell surface carbohydrates might be an important clue to explain the mechanisms of organ-specific cancer metastasis. Specific distribution of glycolipids were also demonstrated on rat cerebrum, small intestine and kidney under various pathological states including diabetes. Histochemical approaches revealed important aspects of the dynamic changes of sugar expression on various tissues and cells.