Exploring the possibilities of genome recombination

针对嗜热古生菌转化效率高的特点，我们探索了进行大规模DNA重组的可能性。以尿嘧啶或色氨酸营养缺陷型突变株为宿主细胞，根据其原生性进行选择。我们能够利用野生型柯达卡氏锥虫的基因组DNA获得原养生物。通过预先插入来自DNA供体的一小段DNA，我们能够使用来自其他高温菌的基因组DNA获得转化子。

Focusing on the high transformation efficiency of the hyperthermophilic archaeon Thermococcus kodakarensis, we explored the possibilities of performing wide-scale DNA recombination. Mutant strains displaying uracil or tryptophan auxotrophy were used as host cells, and selection was based on their prototrophy. We were able to obtain prototrophs using the genomic DNA of wild-type T. kodakarensis. By inserting a small DNA fragment from the DNA donor beforehand, we were able to obtain transformants using genomic DNA from other hyperthermophiles.