Development of highly precise mass spectrometric analytical system for creation of nutrimetabolomics

本研究尝试建立一种基于局部组织微区二维分子行为的fbod因子功能评价新方法。DI-MS系统能够同时显示外源性食物因子和内源性代谢物。由于质谱对各种分子的选择性和检测限取决于其电离率，因此我们进行了有助于食物因子和内源代谢物电离的基质的筛选和优化。所获得的矩阵使我们能够二维可视化的绿色茶儿茶素表没食子儿茶素表没食子儿茶素在组织微区后，口服给药的表没食子儿茶素。这些结果表明，我们提出的质谱系统可能有助于建立一种高度精确和可靠的技术，以评估fbod因子的潜在功能，从有效性和安全性的角度来看。

Herein, we attempted to establish a novel evaluation method of function of fbod factor based on two-dimensional molecular behavior in local tissuemicro-regions, by developing an advanced MAI. DI-MS system capable of visualizing of bothexoge nous food factors and endogenous metabolites simultaneously. Because MS selecti vityand detection limit against various molecules are dependent on its ionization rate, we performed the screening of matrix, which can aid the ionization of both food factor and endogenous metabolites, and its optimization. The obtained matrix enabled us totwo-dimensionally visualize the green tea catechin EGCG in tissue micro-regions after theoral administration of EGCG and endogenous metabolites in response to EGCG. Theseresults suggest that our proposed MS system may contribute to the establishment of highlyprecise and reliable technique fbr evaluating the potential function of fbod factor in view ofefficacy and safbty.