Mechanisms of spinal cord regeneration by transplantation of matured animal-derived somatic stem cells

大鼠脊髓在Th9-10水平上受到挤压损伤。培养的骨髓基质细胞（BMSCs）在损伤后1周（1组）、2周（2组）和4周（3组）开始移植。将细胞注入脑脊液。第一次注射后4周固定大鼠。三组的BBB评分明显高于对照组。三组在星形胶质细胞缺失的损伤处生长出大量再生轴突。星形胶质细胞缺失区由结缔组织组成。轴突被雪旺细胞包围。骨髓间充质干细胞移植是治疗亚急性和慢性脊髓损伤的有效方法。将骨髓分离的单核细胞注射到脊髓损伤大鼠的脑脊液中。运动改善和组织修复得到了保证。培养脉络膜丛上皮细胞并将其直接移植到大鼠脊髓病变中。再生轴突沿着移植细胞生长，这些细胞与宿主组织结合。

The spinal cord was crush-injured at Th9-10 level in rats. Transplantation of cultured bone marrow stromal cells (BMSCs) began 1 (group 1), 2 (group 2), and 4 weeks (group 3) after injury. Cells were injected into the CSF. Rats were fixed at 4 weeks after the initial injection. BBB scores were significantly higher in the three groups than the control. Numerous regenerating axons grew out through the lesion devoid of astrocytes in the three groups. Astrocyte-devoid areas are composed of connective tissues. Axons were surrounded by Schwann cells. BMSC transplantation is effective for subacute and chronic spinal cord injuries. Mononuclear cells isolated from the bone marrow were injected into the CSF of spinal cord-injured rats. Locomotor improvement and tissue repair were secured. Choroid plexus epithelial cells were cultured and transplanted directly into the spinal cord lesion of rats. Regenerating axons grew out along engrafted cells that were integrated into the host tissues.