Interaction between vacuolar H+ ATPase and lymphocytic cholinergic system in regulation of immune function

本文研究了液泡型H ~+ ATP酶（V-ATP酶）与淋巴细胞胆碱能系统在免疫调节中的相互作用。除了mediatophore，这是一个同源寡聚体的16 kDa的亚基的蛋白脂亚基c的V0部门的V-ATP酶，几个亚基（a，B，c，d，e）的表达的V0部门的mRNA，这是一个疏水性的跨膜部门的V-ATP酶，在两个人白血病T细胞系CCRF-CCRF-CEM和MOLT-3，以及人和小鼠的MNL。T细胞活化诱导V-ATP酶亚基表达略有增加。抗介体siRNA可降低介体mRNA的表达和乙酰胆碱（ACh）从T细胞的释放，而不降低乙酰胆碱合成酶胆碱乙酰转移酶的表达。这些结果表明，T细胞表达V-ATP酶V0组分的多个亚基，并且T细胞的乙酰胆碱释放受V-ATP酶的调节。

We investigated interaction between vacuolar type H+ ATPase (V-ATPase) and lymphocytic cholinergic system in regulation of immune activity. In addition of mediatophore, which is a homooligomer with a 16-kDa subunit homologous to proteolipid subunit c of the V0 sector of V-ATPase, expression of several subunits (a,b,c,d,e) of the V0 sector mRNAs, which is a hydrophobic transmembrane sector of the V-ATPase, were detected in two human leukemic T cell lines CCRF-CCRF-CEM and MOLT-3, and MNLs of human and mouse. T cell activation induced a slight increase in expression of V-ATPase subunits. Anti-mediatophore siRNA decreased mediatophore mRNA expression and release of acetylcholine (ACh) from T cells, without reducing expression of choline acetyltransferase, a synthesizing enzyme of ACh. These findings indiucate that T cells express several subunits of V-ATPase V0 component, and acetylcholine release from T cells is regulated by V-ATPase.