Comprehensive investigation of tension-sensitive factors that related to changes in cytoskeleton and cell adhesion in trabecular meshwork cells

我们制作了一种装置，对培养的细胞施加静水压力，即使在对照条件下，细胞形态也发生了变化，数据无法分析。经Y-27632处理后，5个与抗氧化功能相关的基因表达上调。其中，过氧化氢酶的上调通过实时RT-PCR证实。Y-27632预处理后，WST-8检测信号增强，DCFDA检测试剂在过氧化氢刺激下信号减弱。总的来说，表明ROCK抑制剂诱导小梁网细胞中抗氧化剂的细胞内表达，从而抑制氧化应激诱导的细胞死亡。

We made a device to add hydrostatic pressure on cultured cells, resulting in changes in cell morphology even in the control condition, and the data were not analyzable. After treatment of trabecular meshwork cells with Y-27632, 5 genes that were related to antioxidant functions were up-regulated. Of those, the up-regulation of catalase was confirmed by realtime RT-PCR. Pretreatment with Y-27632 increased the signal of WST-8 assay and decreased the signal of DCFDA test reagent under stimulation with hydrogen peroxide. Collectively, it was indicated that ROCK inhibitor induced intracellular expression of antioxidants in the trabecular meshwork cells, and thereby suppressed oxidative stress-induced cell death.