Remineralization of preliminarily carious by enamel proteins with artificial body fluids

背景：人类牙齿的治疗已经从外科手术转向更保守的方法，以抑制或防止病变进展。增加牙釉质矿化在这方面是至关重要的。一个潜在的困难是牙釉质最外层的优先矿化会阻碍整体矿化。我们描述了一种增加牙釉质矿化潜力的策略。提取的人类前磨牙牙釉质暴露在高浓度过氧化氢的激励源。样品在37℃的人工唾液中保存1周。采用台式x射线微ct系统对样品进行了矿物密度评价。由于渗透过氧化氢的活化，带电荷的氧自由基使釉质矿化程度较低和较高的部分之间的矿物分布发生极化。深层牙釉质区域的能量吸收动力学表明，在不限制牙釉质整体矿化的情况下，优先矿化进入该区域。随后增加的矿化，即使在致密矿化外层的牙釉质，也实现了。这种增加的矿化可以增强结构对酸性变质的抵抗力。本研究是在牙医学修复和修复中开发新应用的主要步骤之一。

Background: Management of human teeth has moved from a surgical to a more conservative approach of inhibiting or preventing lesion progression. Increasing enamel mineralization is crucial in this regard. A potential difficulty is the preferential mineralization of the outermost portion of the enamel that can prevent overall mineralization. We describe a strategy for increasing the mineralization potential of dental enamel. Extracted human premolar teeth enamel were exposed to a high concentration of hydrogen peroxide with an energizing source. Samples were stored in artificial saliva at 37uC for 1 wk. A desktop X-ray micro-CT system was used to evaluate the mineral density of samples. Mineral distribution was polarized between the lower and the higher mineralized portion of enamel by charged oxygen free radicals due to activation of permeated hydrogenperoxide. The kinetics of energy absorption in the deeper enamel region demonstrated improvement of preferential mineralization into the region without restricting overall mineralization of the enamel. Subsequent increasing mineralization, even in the dense mineralized outer portion of enamel, was also achieved. This increased mineralization may promote resistance to acidic deterioration of the structure. The present study is one of the primary steps towards the development of novel application in reparative and restorative dentistry.