Center for Excellence In-Host Pathogen Interactions

项目摘要：这是一个行政补充建议，以回应NIGMS通知编号（不是- GM-21-029）。这项补充建议的目的是取代过时的共聚焦显微镜在 Imaging Core配备最先进的Leica Stellaris 5共聚焦显微镜，支持并扩展 宿主病原体COBRE计划我们现有的16岁蔡司510 Meta共聚焦显微镜， 2016年达到使用寿命，基于旧技术，并开始出现维修问题。 因此，COBRE研究人员无法采用可能使用的成像方法， 当前最先进的显微镜中存在的技术。用Leica Stellaris 5替换该仪器 共聚焦显微镜将确保研究人员拥有支持和推进所需的技术 他们的研究目标。这些项目的总体目标是了解免疫过程， 在由病毒、细菌和真菌引起的疾病中， 寄生虫感染所有项目都需要对标记有 荧光染料在固定和活样品中的激发波长范围为405 nm至633 nm。不像 成像核心中现有的共焦显微镜，这是一个有效的3通道系统， 与传统的PMT检测器相比，Stellaris 5共焦显微镜是基于棱镜的四通道系统， 配备超灵敏的Power HyD S探测器，并提供一系列先进的成像功能 包括超分辨率成像。它将允许调查人员对标记有四个或更多的样本进行成像 荧光标记，包括染料如DAPI，其不能使用我们现有的仪器可视化。的 Stellaris 5显微镜将集成到我们已经功能齐全的成像核心中， 成像设备，并将利用现有的基于网络的调度，技术支持， 数据存储和共享，系统合同支持，以及由 显微镜这种仪器的获得将建立在我们非常成功的COBRE的基础上， INBRE和DaCCoTA CTR程序。Stellaris 5共焦，具有超分辨率和其他先进的 能力将提高宿主病原体COBRE研究人员的生物医学科学研究能力。它将 还扩展了发育和疾病表观基因组学研究人员的研究能力 COBRE、隶属于DaCCoTA CTR的研究人员以及UND SMHS的其他研究人员， UND校园，并在区域研究社区。

Project Summary: This is an administrative supplement proposal in response to NIGMS Notice number (NOT- GM-21-029). The purpose of this supplement proposal is to replace an outdated confocal microscope in the Imaging Core with a state-of-the-art Leica Stellaris 5 confocal microscope to support and extend the goals of the Host-Pathogen COBRE program. Our existing 16-year old Zeiss 510 META confocal microscope, which reached its end of service life in 2016, is based on old technology and is beginning to experience repair issues. As a result, COBRE investigators are unable to undertake imaging approaches that are possible using the technology that exists in current state-of-the-art microscopes. Replacing this instrument with a Leica Stellaris 5 confocal microscope will ensure that investigators have the technology they require to support and advance their research goals. The overall goals of these projects are to understand the immunological processes and mechanisms that underly a range of host-pathogen interactions in diseases caused by viral, bacterial, and parasitic infections. All projects require high resolution, multichannel imaging of samples labeled with fluorochromes having excitation wavelengths ranging from 405 nm to 633 nm in fixed and live samples. Unlike the existing confocal microscope in the Imaging Core, which is effectively a 3-channel system with conventional PMT detectors, the Stellaris 5 confocal microscope is a prism-based four channel system, equipped with ultrasensitive Power HyD S detectors and offering an array of advanced imaging features including superresolution imaging. It will allow investigators to image samples labelled with four or more fluorescent labels, including dyes such as DAPI, which cannot be visualized using our existing instrument. The Stellaris 5 microscope will be integrated into our already functional Imaging Core in association with other imaging equipment and will take advantage of existing web-based scheduling, technical support, web-based data storage and sharing, system contract support, and a microscopic imaging service supported by telemicroscopy. Acquisition of this instrumentation will build institutionally on our very successful COBRE, INBRE, and DaCCoTA CTR programs. The Stellaris 5 confocal with superresolution and other advanced capabilities will advance biomedical science research capacity by Host-Pathogen COBRE investigators. It will also extend the research capacity of investigators affiliated with Epigenomics in Development and Disease COBRE, investigators affiliated with the DaCCoTA CTR, and other investigators in the UND SMHS, on the UND campus, and in the regional research community.