The T cell genome in 3D: linking chromatin structure to cellular function

适应性免疫细胞激活导致特定细胞功能的获得和长期维持，从而实现对感染的有效免疫控制。这项建议利用先进的细胞和基因组方法，结合高分辨率显微镜和尖端计算生物学，旨在解决我们对基因组3D结构和靶向生化修饰的改变如何影响细胞特异性基因核定位以及这如何调控与免疫细胞激活相关的基因表达变化的知识中的主要空白。一个结果将是识别新的分子机制，这些机制将在细胞生物学中具有广泛的适用性，并为药物开发提供新的靶点。

Adaptive immune cell activation results in the acquisition and long term maintenance of specific cellular function that enables efficient immune control of infections. Using advanced cellular and genomic approaches, combined with high-resolution microscopy and cutting edge computational biology, this proposal aims to address major gaps in our knowledge about how alterations in genomic 3D architecture and targeted biochemical modifications impact cell specific gene nuclear positioning and how this regulates changes in gene expression associated with immune cell activation. An outcome will be identification of novel molecular mechanisms that will have broad applicability across cellular biology, and provide novel targets for drug development.