MYOCARDIAL CELL ANALYSIS SYSTEM (MCAS)

应用最新技术同时测量环境质量 细胞内离子浓度以及细胞膜电位 与心肌细胞节律性一起对 增进对心脏疾病病理生理学的认识。通过 这样做，将有新的方法来预防和 心脏病的治疗性干预。为了满足这一需求，一个 将开发心肌细胞分析系统(MCAS)。MCAS是 无中断、交钥匙系统，可实时、同时 测量心肌细胞搏动频率、细胞膜电位(或 电活动)和至少两个细胞内离子 水中的浓度(Na+加氯；或钙加氯) 心肌细胞。MCAS使上述蜂窝功能能够 A)使用倒置显微镜对培养的心肌细胞进行测量 (体外MCAS-IV系统)，或b)使用 耦合探针(原位MCAS-IS探针)。在这个第一阶段的项目中， 将开发一种利用荧光光子的光纤原型 离子灵敏荧光团和外差激光的计数 散布。信号处理将包括时频和时间尺度 分析(小波)。这台MCAS样机将进行校准和测试 在培养的心肌细胞和原位心脏模型中。同相 MCAS将被打包成一个交钥匙系统，直接 安装在大多数商用显微镜上。 建议的商业应用 该系统将被用来研究细胞的异常 心脏病。该系统还适用于评估大多数类型的 许多细胞的生物动力和细胞内离子动力学 类型。

The application of the latest technologies to simultaneously measure the concentration of intracellular ions, as well as cell membrane potential together with myocardial cell rhythmicity is of central importance to advance the understanding of the pathophysiology of heart diseases. By so doing, novel approaches will be forthcoming for prophylactic and therapeutic intervention of heart disease. To address this need, a Myocardial Cell Analysis System (MCAS) will be developed. The MCAS is a non-disruptive, turnkey system that in real-time, simultaneously measures myocardial cell beat rate, cell membrane potential (or electrical activity) and a minimum of two intracellular ion concentrations (Na+ together with Cl-; or Ca2+ together with Cl-) in cardiac myocytes. The MCAS enables the above cellular functions to be measured either, a) in cultured myocytes using an inverted microscope (an in vitro MCAS-IV system), or b) in isolated perfused hearts using a coupling probe (an in situ MCAS-IS probe). In this Phase I project, a fiberoptic prototype will be developed utilizing fluorescence photon counting of ion sensitive fluorophores and heterodyne laser light scattering. Signal processing will include time-frequency and time-scale analyses (wavelet). This prototype MCAS will be calibrated and tested in cultured cardiac myocytes and in an in situ heart model. In Phase II, the MCAS will be packaged into a turn-key system that directly attaches to most commercial microscopes. PROPOSED COMMERCIAL APPLICATION This system will be used in investigating the cellular abnormalities of heart diseases. The system is also applicable to assess most types of biological motilities as well as intracellular ion dynamics of many cell types.