PATTERNED IMMOBILIZATION OF BIOTIN ON GLASS

这项研究提供了一种解决关键和持久问题的方法 及时开发可靠的生物传感器系统的障碍 基因序列、癌症、传染性多个标志物的测量 疾病和有毒物质。这项拟议研究的总体目标是 计划(SBIR第一阶段、第二阶段和第三阶段)是开发和商业化制造 用于与健康相关的可用的、改进的多分析生物传感器技术 微量分析。该方法将涉及SurModics的扩展 将光电链接技术应用到表面的普通钝化和 传感器表面上结合基团的图案化固定。这个 模型传感器表面将用两亲性聚合物进行玻璃钝化 或蛋白质，并与广泛使用的生物素--亲和素结合形成图案 一对。生物素将(1)共价结合到钝化表面 通过掩模照明以200微米点为单位的光化学，(2) 饱和了其非常高的亲和力，多位点结合蛋白X- 亲和素(亲和素、NeutravidinTM或Streptavidin)通过亲和结合 原始原料，和(3)负载的固定化X-亲和素斑点 用所需的生物素化寡核苷酸喷墨打印 耐药结核分枝杆菌 检测/识别。因此，这种方法提供了一种创新的 双自由基光化学与高亲和力特异性的结合 结合对，以提供具有成本效益的涂层技术 图案化的多分析物生物传感器表面。 建议的商业应用 这项合成工作预计将在第一阶段产生两个新的 供研究和工业界使用的适销对路的产品： (1)光反应玻璃/硅片和(2)钝化生物素化 玻璃/硅晶片。具有成本效益的生产的潜力 生物分子兼容的图案化多分子传感器表面，将被 在第二阶段证明，对生物传感器具有公认的巨大价值 工业。

This research presents an approach to resolving a critical and enduring obstacle to developing dependable biosensor systems for the timely measure of multiple markers for gene sequences, cancers, infectious diseases, and toxic agents. The broad goal of this proposed research program (SBIR Phases I, II and III) is to develop and make commercially available, improved multianalyte biosensor technology for health-related microanalyses. The approach will involve the extension of SurModics PhotoLink technology into general passivation of the surface and patterned immobilization of binding groups on the sensor surface. The model sensor surface will be glass passivated with amphiphilic polymers or proteins and patterned with the widely useful biotin--avidin binding pair. Biotin will be (1) covalently bound to the passivated surface photochemically in 200 mum dots by illumination through a mask, (2) saturated with its very high affinity, multi-site binding protein X- avidin (avidin, NeutravidinTM, or streptavidin) by affinity binding from crude source material, and (3) the immobilized X-avidin dots loaded by ink-jet printing with the desired biotinylated oligonucleotides for antibiotic resistant Mycobacterium tuberculosis (TB) detection/identification. This approach thus present an innovative combination of diradical photochemistry and high-affinity specific binding pairs to provide a cost-effective coating technology for patterned multianalyte biosensor surfaces. PROPOSED COMMERCIAL APPLICATION This synthetic work is expected to generate in Phase I, two new marketable products for use by the research and industrial communities: (1) Photoreactive glass/silicon wafers and (2) Passivated biotinylated glass/silicon wafers. The potential for cost-effective production of biomolecule-compatible patterned multianlyte sensor surfaces, to be demonstrated in Phase II, has a recognized large value to the biosensor industry.