13 ERA-CAPS The role of the N-end rule pathway in controlling plant response to the environment

在不可预测的生长条件下，对植物进行操纵以提供产量的稳定性，对于应对气候变化的影响至关重要，因为气候变化增加了农业环境的不确定性。通过蛋白水解选择性和有条件地去除调节蛋白正成为植物的主要调节原理。n -环境项目的目的是提供一个完整的机制理解的作用的n端规则途径的靶向蛋白水解在控制植物对环境的反应。为了实现这一目标，我们选择了代表国际领先的欧洲团队的合作伙伴，他们在该途径的多个方面都有经验，包括途径的生化基础、缺氧和植物发育、应激和代谢信号的转录调节。该项目将汇集四个机构的六个研究小组，他们在基础分子植物科学、生物化学和化学方面具有互补的专业知识。n -环境联盟的研究方案将通过由四个联盟伙伴（三个由环境研究计划署资助，一个联合实验室）执行的六个相互关联的工作包来实现。这些工作包的目标与三个基本问题有关，这些问题是在整合了最近由联盟成员发现的靶向蛋白水解的n端规则途径作为植物发育和对环境响应的主要调节剂的作用后产生的。问题1:n端规则通路的蛋白质底物和酶组分是什么？问题2:Nendrule通路是如何整合到细胞信号通路中的？问题3:n端规则通路在植物对生物和非生物环境的响应中发挥了多大的作用？拟议的研究具有高度创新的可测量的结果，解决了植物生物学这一新发现的领域，并将揭示：调节蛋白质稳定性的新机制，环境压力传感的新机制，蛋白质在压力传感中的新功能，然后结束规则在欧盟主要作物西红柿中的重要性。这个快速发展的植物分子科学领域是由n - environment成员（包括底物的发现、进入n端规则通路的方法、途径的生化成分）领导的，加上联盟内大量独特资源的可用性，使得n -环境的提议非常新颖和及时。这一合作项目的协同价值将是开发和利用对农业至关重要的高度新颖的植物生物学领域，欧洲有能力通过ERA-CAPS资助在这一领域走在世界前列。

Manipulation of plants to provide stability of yield under unpredictable growth conditions will beessential to respond to the effect of climate change in increasing the uncertainty of the agriculturalenvironment. Selective and conditional removal of regulator proteins by proteolysis is emerging as amajor regulatory principle in plants. The aim of the N-vironment project is to provide a completemechanistic understanding of the role of the N-end rule pathway of targeted proteolysis incontrolling plant responses to the environment. To achieve this goal consortium partners have beenselected that represent internationally leading European teams with experience in the pathwaysmultiple facets, including the biochemical basis of the pathway, hypoxia and plant development,transcriptional regulation of stress and metabolic signalling. The project will bring together sixresearch groups with complementary expertise in fundamental molecular plant science,biochemistry and chemistry, in four institutions. The research programme of the N-vironmentconsortium will be achieved through six inter-related Work Packages carried out by the fourconsortium partners (three funded by ERA-CAPS, one associated laboratory). The objectives of thesework packages are related to three fundamental questions that arose after integration of the recentdiscoveries by consortium members of the role of the N-end rule pathway of targeted proteolysis as amajor regulator of plant development and response to the environment. Question 1: What are theprotein substrates and enzymatic components of the N-end rule pathway? Question 2: How is the Nendrule pathway integrated into cellular signaling pathways? Question 3: What is the extent of therole of the N-end rule pathway in plant response to the biotic and abiotic environment? The proposedresearch has highly innovative measurable outcomes that address this newly discovered area of plantbiology, and will uncover: New mechanisms regulating protein stability, new mechanisms ofenvironmental stress sensing, new functions for proteins in stress sensing, the importance of theN-end rule in a key EU crop, tomato. This fast-developing area of plant molecular science is led byN-vironment members (including discovery of substrates, methods of entry into the N-end rulepathway, biochemical components of the pathway), and together with the availability of a largenumber of unique resources within the consortium, makes the N-vironment proposal highly novel andtimely. The synergistic value of this collaborative programme will be the development andexploitation of a highly novel area of plant biology of key importance to agriculture, in which Europehas the capacity to take a world lead through ERA-CAPS funding.