ACTH AND RELATED PEPTIDES

我们的目标是研究结构-活性关系 促肾上腺皮质激素（ACTH）、促脂素（LPH）、内啡肽（EP）、促黑素 （MSH）和相关肽。 计划分离ACTH、Beta-LPH、 从大象脑垂体提取β-EP和相关肽， 确定它们的氨基酸序列。 一旦知道了大象的结构 固相法合成ACTH、β-EP及相关肽 法 还计划合成角鲨和两栖动物ACTH。 当合成肽变得可用时，它们的生物学特性 被调查。 α-ACTH-（7-38）[促肾上腺皮质激素抑制肽（CIP）]， ACTH的抑制剂，是一种弱拮抗剂。 希望能 合成具有更高抑制活性CIP类似物。 同样 计划合成β-EP-（1-27）的类似物，[β-EP抑制 肽（BetaEIP）]，天然存在的β-EP诱导的抑制剂 镇痛，具有较高的抑制活性。 用于开发灵敏和特异的放射免疫测定（RIA）， 大鼠和人的γ-LPH，合成这两种γ-LPH是必要的 固相法。 已知ACTH和相关肽不表现出三维结构， 水中的结构。 计划调查是否存在一个 人ACTH、β-MSH和 α-MSH的酶促反应的差吸收光谱法 荷尔蒙

Our objective is to study the structure-activity relationship of corticotropins (ACTHs), lipotropins (LPHs), endorphins (EPs), melanotropins (MSHs) and related peptides. It is planned to isolate ACTH, Beta-LPH, Beta-EP and related peptides from elephant pituitary glands and to determine their amino acid sequences. Once the structure is known elephant ACTH, Beta-EP and related peptides will be synthesized by the solid-phase method. It is also planned to synthesize dogfish and amphibian ACTHs. When the synthetic peptides become available, their biological properties are investigated. Alphah-ACTH-(7-38) [corticotropin-inhibiting peptide (CIP)], the naturally occurring inhibitor to ACTH, is a weak antagonist. It is hope to synthesize analogs of CIP with higher inhibitory activity. Similarly, it is planned to synthesize analogs of Betah-EP-(1-27), [Beta-EP inhibiting peptide (BetaEIP)], the naturally occurring inhibitor to Beta-EP-induced analgesia, with higher inhibitory activity. For the development of sensitive and specific radioimmunoassays (RIAs) for rat and human Gamma-LPH, it is necessary to synthesize these two Gamma-LPHs by the solid-phase method. ACTH and related peptides are known to exhibit no three dimentional structures in water. It is planned to investigate the presence of a tertiary structure in aqueous solutions of human ACTH, Beta-MSH and Alpha-MSH by difference absorption spectroscopy of enzymic digests of the hormones.