IMPROVED GLUCOSE BIOSENSORS

拟议的工作涉及设计和测试新的酶为基础的 电流型葡萄糖传感器， 葡萄糖氧化酶的黄素氧化还原中心和电极， 供体-受体中继网络化学结合到高度柔性的， 不溶性高分子量聚合物。 稳定性的提高， 选择性将通过使用保护性聚合物来实现 涂层和新的酶固定化方法。 优化 传感器应该在以下领域具有广泛的技术应用， 作为临床实验室，兽医保健，研究 实验室和食品工业。 使用保护性聚合物 防止传感器在生物流体中结垢的涂层，以及 电子转移介体共价连接到不溶性 聚合物以防止它们从电极扩散， 一种可重复使用的体外血糖监测仪的开发（用于 糖尿病患者的自我监测）似乎是可行的。 聚合物 中继系统也适合于针型探测器 对许多研究应用都很有用。

The proposed work involves the design and testing of new enzyme-based amperometric glucose sensors where electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible, insoluble, high molecular weight polymer. Improvements in stability and selectivity will be pursued through the use of protective polymer coatings and novel enzyme immobilization procedures. The optimized sensors should have widespread technological applications in areas such as the clinical laboratory, veterinary health care, the research laboratory, and the food industry. With the use of protective polymer coatings to prevent fouling of the sensor in biological fluids, and electron transfer mediators which are covalently attached to an insoluble polymer to prevent their diffusion away from the electrode, the development of a reusable in vitro blood glucose monitor (for self-monitoring by diabetic patients) appears feasible. The polymeric relay systems are also amenable to needle-type probes which could be useful for many research applications.