Decoding microtubule remodelling in sperm production

所有真核细胞都具有动态的微管（MT）细胞骨架，需要不断重塑以满足其许多基本的细胞功能。新出现的数据表明，对MT表面（微管蛋白代码）的修改可能作为重塑的指导性路标。该项目旨在定义微管蛋白代码的一个基本组成部分，谷氨酰化，并定义它如何指导MT切断。明确MT切断酶FIGNL1和关键MT谷氨酰化酶（CCP1、CCP5和TTLL1）的细胞功能。除了与真核生物物种的细胞生物学相关外，还将利用精子产生作为模型系统来产生见解，从而告知可育精子形成的机制。

All eukaryotic cells possess a dynamic microtubule (MT) cytoskeleton, which requires constant remodelling to satisfy its many essential cellular roles. Emerging data suggests modifications to the MT surface (the tubulin code) may act as instructional signposts for remodelling. This project aims to define a fundamental component of the tubulin code, glutamylation, and define how this directs MT severing. It also aims to define the cellular functions of MT-severing enzyme FIGNL1 and key MT glutamylation enzymes (CCP1, CCP5 and TTLL1). Insights will be generated using sperm production as a model system and will thus inform the mechanisms by which fertile sperm are built, in addition to being relevant to cell biology across eukaryotic species.