GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

作为绘制和测序人类基因组图的努力的一部分，我们一直在 参与了一项正在进行的努力，以开发一份多位点遗传图谱 以小鼠为模式的哺乳动物系统。这项工作的大部分都是 通过对两个基因的后代进行遗传分析来完成 杂交，种间回交和亚种间回交。DNA来自于 这些杂交组合的后代已经被分型为各种多态 允许将未知标记映射到特定位置的参考基因座 在连锁图上。这些研究已经导致了染色体 大量基因的定位，包括各种基因在 神经组织，细胞周期蛋白基因，热休克蛋白，转录 调节因子、微管相关蛋白基因和同源异型盒基因。 这些基因中有几个位于或接近已知的小鼠突变，因此 是这些突变的潜在候选者。因此，有一个基因是特定的 在大脑中的表达被定位在缺陷性神经突变附近 在突变的缺陷型小鼠中显示为缺失。另一个基因编码一种 软骨的主要结构成分位于软骨(Cmd)附近 基质缺乏症)突变，cmd小鼠被证明有一个7b 这个基因的缺失。

As part of the effort to map and sequence the human genome, we have been involved in an ongoing effort to develop a multilocus genetic map of the mouse as the model mammalian system. Most of this work has been accomplished by the genetic analysis of the progeny of two genetic crosses, an interspecies and an intersubspecies backcross. DNAs from the progeny of these crosses have been typed for a variety of polymorphic reference loci to permit mapping of unknown markers to specific positions on the linkage map. These studies have resulted in the chromosomal mapping of a large number of genes including various genes expressed in nervous tissue, cyclin genes, heat shock proteins, transcriptional regulators, microtubule associated protein genes and homeobox genes. Several of these genes map at or near known mouse mutations and therefore are potential candidates for these mutations. Thus, one gene specifically expressed in brain was mapped near the coloboma neurological mutation and shown to be deleted in mutant coloboma mice. Another gene encoding a major stuctural component of cartilage was mapped near the cmd (cartilage matrix deficiency) mutation, and cmd mice were shown to have a 7 b deletion of this gene.