IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

我们在这个项目中的目标是研究 具有MHC I类分子的自身和抗原肽 不同的系统，并在三维空间评估这些相互作用 基于结晶学MHC结构的计算机建模研究。是这样的 研究允许我们检查潜在的生物化学规则 抗原肽与MHC分子的相互作用 免疫反应的启动。此外，它们还提供了 理解蛋白质如何结合小分子这一更大问题的框架 分子量多肽配体。 过去一年的主要成就包括 多肽相互作用的定量动力学测定法 与纯化的MHC分子进行实时比较，并对其进行广泛分析 大量多肽的结合。自我的表征 为多肽的大量生产提供了基础 MHC/多肽复合物的分子模型，并允许更详细的 对有助于稳定结合的分子规则的描述。

Our objectives in this project have been to examine the interaction of self and antigenic peptides with the MHC class I molecule using several different systems and to evaluate these interactions in three dimensional computer modeling studies based on crystallographic MHC structures. Such studies permit us to examine underlying biochemical rules that govern the interaction of antigenic peptides with MHC molecules, a central role in the initiation of the immune response. In addition they provide a framework for understanding the larger question of how proteins bind small molecular weight peptide ligands. Major accomplishments in the past year include the development of quantitative kinetic assays for measurement of the interaction of peptides with purified MHC molecules in real time, and the extensive analysis of the binding of a large number of peptides. Characterization of self peptides has provided the basis for the production of a large number of molecular models of MHC/peptide complexes and permits the more detailed description of the molecular rules that contribute to stable binding.