STUDIES ON IMMUNE REGULATION IN TOXOPLASMOSIS

该项目的总体目标是分析免疫反应， 弓形虫，以确定哪些细胞免疫成分， 寄生虫靶抗原参与感染的控制， 免疫受损的宿主中的分解。 今年在以下领域取得了进展： A. 证明针对T.弓形虫感染 细胞 来自接种疫苗的小鼠的CD 8+细胞显示裂解感染的或未感染的细胞。 抗原脉冲的体外巨噬细胞。 抗原部分被部分地 纯化的，其含有适当的靶抗原。 B。 T细胞亚群再激活要求的调查。 T.在慢性感染的小鼠中， 主要是CD 8 + T细胞功能的丧失。 C. MAIDS -弓形虫病模型的建立。 小鼠感染 逆转录病毒混合物（MAIDS）被证明可以降低对 攻击感染并引起潜伏感染的部分再激活。

The overall aim of this project is to analyze the immune response to Toxoplasma gondii in order to define which cellular immune components and parasite target antigens are involved in the control of infection and its breakdown in immunocompromised hosts. Progress was made this year in the following areas: A. Demonstration of CD8+ cytolytic activity against T. gondii infected cells. CD8+ cells from vaccinated mice were shown to lyse infected or antigen pulsed macrophages in vitro. An antigenic fraction was partially purified which contains the appropriate target antigens. B. Investigation of T cell subset requirements for reactivation. Reactivation of T. gondii in chronically infected mice was shown to be due primarily to a loss in CD8+ T cell function. C. Establishment of a MAIDS - toxoplasmosis model. Infection of mice with a retrovirus mixture (MAIDS) was shown to reduce susceptibility to challenge infection and cause partial reactivation of latent infection.