SEQUENCE OF THE SIXTH ROTAVIRUS GENE

本发明还公开了一种新的VP 6基因的核苷酸序列， 两种亚组I动物轮状病毒（长尾猴轮状病毒SA-11和 牛轮状病毒RF）和一种亚组II人轮状病毒（Wa）， 以前确定的。 推导氨基酸的比较 序列表明高度同源性;超过90%的 亚群I和亚群II之间氨基酸保守 轮状病毒 在本研究中，我们推导了VP 6的氨基酸序列， 蛋白质的4个额外的轮状病毒菌株，每一个具有不同的 亚组特异性：人轮状病毒株1076（亚组I）， 猪轮状病毒Gottfied株（亚组（II），马轮状病毒 染色H-2（非亚组I或II）和马轮状病毒株 FI-14（亚组I和II）。 这使我们能够比较VP 6 具有双重或未知亚群的轮状病毒的氨基酸序列 与属于亚组I或II的轮状病毒的反应性。 采用这种方法是为了鉴定表位 负责亚组特异性。 此外，重要性 VP 6蛋白的三聚体构象与亚组 表位进行了研究。 不同毒株的大多数VP 6基因含有独特的AUC。 与此相反，H-2株的VP 6基因含有额外的AUG 在残基132-134处，这导致第二个 VP 6蛋白。 两种VP 6产物均呈现三聚体构象， 成熟的病毒体。

The complete nucleotide sequence of the sixth gene encoding VP6 of two subgroup I animal rotavirus (vervet monkey rotavirus SA-11 and bovine rotavirus RF) and one subgroup II human rotavirus (Wa) was determined previously. Comparison of the deduced amino acid sequences indicated a high degree of homology; more than 90% of the amino acids were conserved between subgroup I and subgroup II rotaviruses. In the present study we deduced the amino acid sequence of the VP6 protein of 4 additional rotavirus stains, each with a different subgroup specificity: human rotavirus strain 1076 (subgroup I), porcine rotavirus strain Gottfied (subgroup (II), equine rotavirus stained H-2 (neither subgroup I nor II) and equine rotavirus strain FI-14 (both subgroup I and II). This allowed us to compare the VP6 amino acid sequences of rotaviruses with dual or no known subgroup reactivity with those of rotaviruses belonging to subgroup I or II. This approach was taken in an attempt to identify epitopes responsible for subgroup specificity. In addition, the importance of the trimeric conformation of the VP6 protein to subgroup epitopes was investigated. Most of the VP6 genes the different strains contain a unique AUG. In contrast, the VP6 gene of H-2 strain contains an additional AUG at residue 132-134, which results in the translation of a second VP6 protein. Both VP6 products assume a trimeric conformation in the mature virion.