MOLECULAR STUDIES OF THE GENOME AND SURFACE OF SCHISTOSOMA MANSONI

本项目的目的是表征非对称表面 与免疫相关的分子，以分析 寄生虫的特定生物学特性 并克隆重要的染色体免疫原。 A. 副肌球蛋白的分子生物学。 副肌球蛋白被证明是 由异常大的转录本编码 cDNA包含 分子的未测序部分被分离沿着 在这些相同的区域。 B。 脂质锚定在精子膜上的证明 proteins. Mr 70 K的四种主要精子表面抗原， 32 k-38 k和18 k被证明是锚定在皮层， 磷脂酰肌醇连接并以膜的形式释放 囊泡 C. 在染色体膜上检测宿主染色体。 使用 特异性抗体，宿主衰变加速因子（RECEPTOR）， 在从猴体内回收的核糖体的膜中发现 和豚鼠。

The aim of this project is to characterize schistosome surface molecules relevant to immunity, to analyze the genome of the parasite in relation to specific biological properties of the organism and to clone important schistosome immunogens. A. Molecular biology of paramyosin. Paramyosin was shown to be encoded by an unusually large transcript. cDNA's comprising unsequenced portions of the molecule were isolated along with peptides in these same regions. B. Demonstration of lipid anchors on schistosomulum membrane proteins. Four major schistosomulum surface antigens of Mr 70K, 32k-38k and 18k were shown to be anchored in the tegument by phosphatidylinositol linkages and released in the form of membrane vesicles. C. Detection of host DAF on the schistosome membrane. Using specific antibodies, host decay accelerating factor (DAF) was identified in the membrane of schistosomes recovered from monkeys and guinea pigs.