ENZYME STRUCTURE

1)色氨酸合成酶晶体的几个数据集 鼠伤寒沙门氏菌已被提纯。精致的结构具有 确认了隧道较低分辨率下的原始观测结果 吲哚在第一次反应中生成的吲哚通过活性部位 第二种酶的活性。正在对突变体进行研究，以期 阐明该隧道和场地相互作用的动态性质 在建筑群里。 2)用X-射线衍射法分析了根霉胃酶的抑制剂络合物。 射线衍射，以期获得更多关于 作用机制。 3)提出了一种结构节点精化方法。 经X-射线衍射法和核磁共振法测定。 4)获得了Y-box蛋白复合体的晶体 非洲爪哇卵母细胞与DNA寡聚体。

1) Several data sets of the tryptophan synthase crystals from Salmonella typhimurium have been refined. The refined structures have confirmed the original observation at lower resolution of a tunnel for the passage of indole generated in the first reaction to the active site of the second enzyme. Mutants are being examined with a view to elucidating the dynamic nature of this tunnel and of the site interaction in the complex. 2) Inhibitor complexes of rhizopus pepsin have been analyzed by X- ray diffraction with a view to obtaining more information concerning the mechanism of action. 3) A method has been developed for the joint refinement of structures determined by X-ray diffraction and by NMR. 4) Crystals have been obtained for the complex of a Y-box protein from Xenopus oocytes with a DNA oligomer.