EVALUATION OF L-TRYPTOPHAN TOXICITY IN MICE

近年来，875例(4例死亡)嗜酸性粒细胞增多症(EMS) 在美国被报道过。似乎与 摄取色氨酸或某些数量的色氨酸。这样做的目的是 研究是确定几个批次中的一个或多个L色氨酸 由疾病控制中心(CDC)提交的可能会导致 雌性B6C3F1冰的毒性。给小鼠灌胃0.5%L色氨酸。 甲基纤维素水，每日0或300毫克/公斤体重，星期一至 星期五21天，30天内灌胃。动物被杀在 第1次治疗后第3、17、30天。多发性血液学和 在给药期间进行临床病理检查。 末次处死时的组织病理学和血液学评价。 这项研究的结果没有提供对小鼠的毒性迹象 可以被解释为与人类所见的EMS有关。B6C3F1鼠标 可能不是敏感型号或测试的批次可能不包含 引起人类EMS的污染物(S)。

Recently 875 cases (4 fatal) of eosinophilia-mylagia-syndrome (EMS) has been reported in the U.S.A. There appears to be an association with ingestion of tryptophan or certain lots of tryptophan. The purpose of this study is to determine if one or more of the several lots of L-tryptophan submitted by the Center of Disease Control (CDC) has the potential to cause toxicity in female B6C3F1 ice. Mice were given L-tryptophan in 0.5% methylcellulose-water at doses of 0 or 300mg/kg B.W. daily from Monday to Friday for 21 days over a 30-day period by gavage. Animals were killed at 3, 17 and 30 days after the first treatment. Multiple hematological and clinical pathology examinations were performed during the dosing with histopathological and hematopoietic evaluations at the terminal sacrifice. The results of this study provided no indication of toxicity in mice that could be interpreted as related to the EMS as seen in man. The B6C3F1 mouse may be not be a sensitive model or the lots tested may not contain the contaminant(s) that causes EMS in man.