CULTURES OF HUMAN CEREBROMICROVASCULAR ENDOTHELIUM: GROWTH AND CHARACTERIZATION

这项研究一直以来都是关于发展和建立的 内皮细胞培养来源于三个微血管组分 人脑。内皮细胞培养的纯度被发现是 超过95%，因为大多数细胞表达人因素 VIII相关抗原，仅偶见胶质细胞染色 纤维酸性蛋白。光学和电子显微镜都表明 单独培养的内皮细胞来源于毛细血管 (EC)或小[(&lt；100MU-m)Es]和大[&gt；100MU-m]小动脉和 小静脉具有相同的外观和生长方式。最大 术后第1天可见内皮细胞的增殖反应 暴露在新鲜介质中。在不同的人之间发现了直接的关系 生长介质的成分和个体的增殖反应 内皮依赖于其来源，表明不同的生长 要求。培养的内皮细胞的特征仍然是 未完成且正在进行中。 然而，成功开发的纯种栽培技术 人脑来源的脑微血管内皮细胞(依赖于 脑组织的可用性)提供了一种体外模型 正常或异常血管内皮细胞特性的研究 人血脑屏障和脑血流量的功能。 由于有毒的人血清而导致的内皮细胞培养的损失防止了 完成这一项目。

This study has been concerned with the development and establishment of endothelial cell cultures derived from three microvascular fractions of human brain. The purity of the endothelial cultures was found to be greater than 95% since most of the cells expressed human Factor VIII-related antigen and only an occasional cell stained for glial fibrillary acidic protein. Both light and electron microscopy showed that the separately cultured endothelium derived either from the capillaries (EC) or small [(<100mu-m)ES] and large [>100mu-m) EL] arterioles and venules exhibited the same appearance and growth pattern. The maximal proliferative response of endothelium was seen on the first day after exposure to fresh medium. A direct relationship was found between various components of growth medium and individual proliferative response of endothelium dependent on its origin indicating different growth requirements. The characterization of the cultured endothelium is still incomplete and in progress. Nevertheless the successfully developed technique for cultivating pure cerebromicrovascular endothelium derived from human brain (dependent on the availability of brain tissue) provides a model in vitro for investigation of endothelial properties related to the normal or altered function of blood-brain barrier and cerebral blood flow in man. The loss of endothelial cultures due to toxic human serum prevented the completion of this project.