BIOCHEMICAL METHODS FOR VASOACTIVE SUBSTANCES

我们已经建立了测量血浆、尿液、组织和 儿茶酚胺前体的脑脊液水平 二羟基苯丙氨酸(DOPA)、儿茶酚胺去甲肾上腺素(NE)、 肾上腺素(E)、多巴胺(DA)和脱氨儿茶酚胺 代谢物二羟基苯甘醇和二羟基苯乙酸 (DOPAC)同时使用高效液相色谱和电化学方法 检测(LCED)。我们还开发了放射免疫分析方法 特异性测定人心钠素1-28型和血管紧张素 II(AII)采用LC/RIA法。 血浆DOPA广泛来源于交感神经末梢，可能 指示儿茶酚胺生物合成中限速步骤的活性。 同时测量DHPG和NE提供了有关 去甲肾上腺素的功能比单独测量任何一种物质都要好。 ANP免疫反应性随盐负荷增加而增加，随站立时间延长而减少 直立，ANP免疫反应减弱。 人类的手臂。

We have established methodology for measuring plasma, urine, tissue, and cerebrospinal fluid levels of the catecholamine precursor dihydroxyphenylalanine (DOPA), the catecholamines norepinephrine (NE), epinephrine (E), and dopamine (DA), and the deaminated catecholamine metabolites dihydroxyphenylglycol (DHPG) and dihydroxyphenylacetic acid (DOPAC) simultaneously using liquid chromatography with electrochemical detection (LCED). We also have developed radioimmunoassay methodology for measuring specifically the 1-28 form of human ANP and measuring angiotensin II (AII) using liquid chromatography with radioimmunoassay (LC/RIA). Plasma DOPA derives extensively from sympathetic nerve endings and may indicate activity of the rate-limiting step in catecholamine biosynthesis. Simultaneous measurement of DHPG and NE provides more information about noradrenergic function than measuring either substance alone. Immunoreactive ANP increases with salt loading and decreases with standing upright, and there is an arteriovenous decrement in immunoreactive ANP in the human arm.