CELLULAR BIOCHEMISTRY STUDIES ON CHEMICAL SELECTED FOR EVALUATION BY NTP

无机或有机金属和金属络合物的作用是 特别感兴趣的NTP，因为他们在饮酒的流行 水和工业过程，用作抗癌药物的成分， 及其不同的靶器官毒性。 在暴露的大鼠或小鼠中研究细胞免疫毒性的机制 氯化汞、硫酸镍或二氯化二茂钛。 组织 这些金属在靶器官中的积累表明， 用于评价靶器官毒性的生化测定法通常 肉眼、显微镜或临床方法。 动物模型的开发 研究费舍尔大鼠白血病揭示了这种疾病的肿瘤标志物， 可以用来区分年龄诱导的和化学增强的 白血病发生 化学性肾损伤后尿酶的变化 作为模型，以预测慢性肾毒性， 问题研究 酶法开发，以提高稳定性，灵敏度， 并启动7-乙氧基香豆素-邻-脱甲基酶的效率， 评估微量啮齿动物中化学品的MFO引发活性 组织.

The effect of inorganic or organic metals and metal complexes is of particulr interest to the NTP because of their prevalence in drinking water and industrial processes, use as constituents in anticancer drugs, and their diverse target organ toxicities. Mechanisms of cellular immunotoxicity were studies in rats or mice exposed to mercuric chloride, nickel sulfate or titanocene dichloride. Tissue accumulation of these metals in target organs indicated the sensitivity of the biochemical assays to evaluate target organ toxicity often preceeded grossgross, microscopic or clinical methods. Development of animal models to study Fischer rat leukemia revealed tumor markers for this disease that can be used to distinguish between age-induced and chemically-enhanced leukemogenesis. Urinary enzyme responses to nephrotoxic chemical insult were evaluated to use as a model to predict renal toxicity in chronic studies. Enzymtic method development to enhance stability, sensitivity, and efficiency of 7-ethoxycoumrin-ortho-demethylase was initiated to evaluate MFO-initiating activity of chemicals in minute amounts of rodent tissue.