TRANSMEMBRANE WATER AND ION MEASUREMENT SYSTEM (TWIMS)

描述(改编自申请者摘要)：利登科技公司提议 结合纳秒相位调频荧光寿命 双向水流量测量系统的测量技术 由Lidon原理开发；跨膜水和离子 测量系统(TWIMS)(Philis，Wong&Yeates，1999)。该系统基于 关于时间变化调制的测量方法 8-氨基-1，3，6-萘三磺酸盐(ANTS)在D2O中的荧光比较 由于D2O和H2O通过膜的双向传输而变成H2O 分区。蚂蚁通过发射397 nm的光进行激发 频率为MHz的正弦调制蓝色LED。这一阶段以及 514 nm处发射的荧光的调制因子是使用 结合互相关技术的双光电倍增管。在……里面 在这个第一阶段的可行性项目中，利顿将结合这些光电技术 提高系统灵敏度和时间分辨率的技术。 在第二阶段，系统将进一步优化和自动化，以增强 TWIMS的新颖性，同时降低生产成本，从而实现盈利 可以制造系统来获得市场竞争力。维护 动态平衡的水在生物膜上的传输是 生命和跨膜转运异常导致器官疾病 和组织，包括肺、肠、肾、眼和脑。这 该系统的目标是一个尚未开发的市场。据预测，这个市场将经历 大幅增长 建议的商业应用：不可用

DESCRIPTION (adapted from applicant's abstract): LiDon Technologies proposes to incorporate a nanosecond phase-frequency modulation fluorescence lifetime measurement technique to a bi-directional water flux measurement system developed by the principals of LiDon; the Trans-membrane Water and Ion Measurement System (TWIMS) (Philis, Wong & Yeates, 1999). The system is based on the measurement of the modulation of the temporal changes in the fluorescence of 8-amino-1,3,6-naphthalene tri-sulfonate (ANTS) in D2O, compared to H2O due to bi-directional transport of D2O and H2O across a membrane partition. ANTS is excited with 397 nm light emitted from a sinusoidally-modulated blue LED at MHz frequencies. The phase as well as the modulation factor of the emitted fluorescence at 514 nm are determined using a dual photomultiplier tube in conjunction with a cross correlation technique. In this Phase I feasibility project, LiDon will incorporate these electro-optical technologies to improve the sensitivity and temporal resolution of the system. In Phase II, the system will be further optimized and automated to enhance the novelty of the TWIMS while reducing its costs of production so that profitable systems can be manufactured to attain market competitiveness. The maintenance of homeostatic water transport across biological membranes is fundamental to life and abnormalities in trans-membrane transport lead to diseases in organs and tissues, including the lungs, intestine, kidneys, eyes, and brain. This system is targeted to a yet untapped market. This market is forecast to undergo substantial growth PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE