METHODS FOR RAPID DETECTION OF CMV

这个正在进行的项目涉及几项研究。努力增加 壳瓶法检测巨细胞病毒的敏感性 继续我们正在研究胸腺嘧啶核苷 类似物，如吲哚-或溴-脱氧尿苷，对CMV生长的影响，以确定 如果他们能加入到我们的培养瓶中， 的文化，以检测CMV在临床标本。海美 溴，或聚凝胺，已被证明有助于人类疱疹病毒6 穿透培养物中的细胞，因此增加了 比色法我们将检查聚凝胺对壳瓶中CMV的影响 培养，看看它是否可以增加壳瓶的灵敏度， 比色法 发现了CMV早期结构蛋白pp 65的检测 是预测CMV疾病进展的快速和敏感的手段。 我们正在检查和评估一组抗pp 65的单克隆抗体 它能识别感染细胞核中表达的抗原决定簇， 外周多形核白细胞和单核细胞， 与我们目前使用的抗pp 65抗体不同。人们希望 这些新的抗体将允许增加检测灵敏度， 临床血液标本中的CMV抗原。 巨细胞病毒可能是艾滋病毒患者肺炎的一个原因。检测 在支气管肺泡灌洗液中发现的由细胞组成的涂片中的CMV可以是 一种快速诊断CMV肺病的方法。我们将研究这样的 使用免疫荧光试剂检测CMV pp 65的涂片， CMV 72 kd立即早期抗原，以确定其临床应用 快速诊断方法。

This ongoing project involves several studies. Efforts to increase the sensitivity of the shell vial assay in detecting cytomegalovirus (CMV) continue. We are in the process of examining the effect of thymidine analogues, such as indo- or bromo-deoxyuridine, on CMV growth to determine if they can be added to our shell vial cultures to enhance the sensitivity of the culture to detect CMV in clinical specimens. Hexadimethrine bromide, or polybrene, has been demonstrated to help human herpes virus 6 penetrate cells in culture and therefore increase the sensitivity of the assay. We will examine the effect of polybrene on CMV in shell vial cultures to see if it can increase the sensitivity of the shell vial assay. The detection of pp65, the early structural protein of CMV, has been found to be a rapid and sensitive means of predicting CMV disease progression. We are examining and evaluating a pool of monoclonal antibodies to pp65 that recognizes the epitopes expressed in the nuclei of infected peripheral polymorphonuclear leukocytes and monocytes and that are different from our currently used antibodies to pp65. It is hoped that these new antibodies will allow an increased detection sensitivity of this CMV antigen in clinical blood specimens. CMV can be a cause of pneumonitis in the patient with HIV. Detection of CMV in smears consisting of cells found in bronchoalveolar lavages can be a rapid means of diagnosing CMV pulmonary disease. We will examine such smears using immunofluorescent reagents for the detection of CMV pp65 and the CMV 72kd immediate early antigen to determine their clinical utility as rapid diagnostic methods.