LCM 220 LASER CAPTURE MICRODISSECTION SYSTEM

该提案要求为购买Pixcell II LCM 220激光捕获显微解剖系统提供资金，该系统将被安置在南卡罗莱纳大学医学院仪器资源设施（IRF）中。IRF是一个核心研究仪器设备，每年有来自南加州大学医学院和附属南卡罗来纳癌症中心、Dom VA和里奇兰纪念医院的150多名教职员工和学生使用。此外，IRF的设备目前被来自南卡罗来纳大学生物系、化学系、工程系和巴鲁克海洋研究所、克莱姆森大学、南卡罗来纳医科大学、本尼迪克特学院和南卡罗来纳州几个行业的几名教师和学生使用。本提案的9名主要参与者代表了南加州大学医学院、南卡罗来纳癌症中心和南加州大学生物系的发育生物学和解剖学、微生物学和免疫学、病理学的教师，他们共同获得了12项NIH研究资助。为位于南卡罗来纳州哥伦比亚的USC医学院IRF收购LCM 220，将为南卡罗来纳州的几名研究人员提供集中访问设备的机会。该工具的行政计划将以过去几年中已成功使用的基金现有行政计划为基础。该系统的行政支持非常出色，承诺为LCM 220提供空间，为协助操作该系统的IRF技术人员提供工资，并支付仪器年度服务合同的费用。激光捕获显微解剖是在过去4-5年中发展起来的一项技术，它提供了一种从复杂组织切片中分离单细胞或同质细胞群的方法，以便随后通过分子或生化技术进行分析。迄今为止，大多数应用都是使用该系统从癌组织中分离出特定细胞，用于随后的DNA、RNA和蛋白质分析。虽然该提案的一些参与者将利用LCM 220分析癌变组织，但其他人建议将该系统的使用扩展到分析从发育和患病的心脏和眼睛组织中分离出来的组织。LCM技术在这些组织中的应用以前没有报道。为了确保LCM是一种适用于这些组织的拟议研究的技术，我们还提供了在Pixcell II LCM 220制造商Arcturus Engineering技术人员的协助下收集的初步数据。

This proposal requests funds for the purchase of the Pixcell II LCM 220 laser capture microdissection system that will be housed in the University of South Carolina School of Medicine Instrumentation Resource Facility (IRF). The IRF is a core research instrumentation facility utilized by more than 150 faculty staff and students from the USC School of Medicine and affiliated South Carolina Cancer Center, Dom VA and Richland Memorial Hospitals each year. In addition, equipment in the IRF is currently used by several faculty and students from the Departments of Biology, Chemistry, Engineering and the Baruch Marine Institute at the University of South Carolina, and Clemson University, the Medical University of South Carolina, Benedict College and several industries around the state of South Carolina. The 9 major participants on this proposal represent faculty from the Departments of Developmental Biology and Anatomy, Microbiology and Immunology, and Pathology in the USC School of Medicine, the South Carolina Cancer Center and the USC Department of Biology and collectively have 12 funded NIH research grants. Acquisition of the LCM 220 for the USC School of Medicine IRF in Columbia, SC will provide centralized access to the equipment for several research personnel in the State of South Carolina. The administrative plan for the instrument will be based on one that is already in place for the IRF which has been used successfully for the past several years. Administrative support for the system is excellent with a commitment to provide the space for the LCM 220, salaries for the IRF technical staff to assist with the operation of the system, and the cost of the annual service contract for the instrument. Laser capture microdissection is a technique that has been developed in the past 4-5 years that provides a method to isolate single cells or groups of homogeneous cells from a complex tissue section for subsequent analysis by molecular or biochemical techniques. The majority of applications to date have used the system to isolate specific cells from cancerous tissues for subsequent analysis of DNA, RNA and protein. While several of the participants in this proposal will utilize the LCM 220 for the analysis of cancerous tissues, others propose to extend the use of the system to analyze tissues isolated from developing and diseased heart and eye tissues. Application of the LCM technology to these tissues has not previously been reported. To insure that LCM is an appropriate technique for the proposed studies with these tissues we also present preliminary data collected with the assistance of the technical staff of Arcturus Engineering, manufacturers of the Pixcell II LCM 220.