Reconstructed Human Oral Tissues

描述（由申请方提供）：I期研究采用原代培养方法，以获得正常人颊上皮（NHBE）细胞的可靠来源。组织培养方法被开发以诱导NHBE细胞分化为三维器官型组织。表征表明，该组织再现了许多组织学，蛋白质表达和天然颊组织的屏障功能特性。最初的实验研究使用NHBE组织预测口腔刺激和经颊给药速率。最后，对组织生产所涉及的成本进行的经济评估表明，NHBE产品似乎在商业上是可行的。 第二阶段的工作计划将扩大第一阶段的结果，以进一步开发NHBE和其他相关的，更复杂的口腔粘膜模型。将优化NHBE培养条件，并确定产生牙龈表型和纳入固有层的条件。将确定预测口腔刺激的方案，并以更全面的方式研究经颊给药组织的使用。最后，研究解决基本的口腔生物学现象，即抗菌肽调节和艾滋病毒感染，将进行。

DESCRIPTION (provided by applicant): Phase I research adapted primary culture methods to obtain a reliable source of normal human buccal epithelial (NHBE) cells. Tissue culture methods were developed to induce the NHBE cells to differentiate into a three-dimensional organotypic tissue. Characterization showed that the tissue reproduced many of the histological, protein expression, and barrier function properties of native buccal tissue. Initial experiments investigated use of the NHBE tissue for predicting oral irritation and transbuccal drug delivery rates. Finally, an economic evaluation of costs involved in tissue production showed that an NHBE product appears to be commercially feasible. The Phase II work plan will expand upon the results of Phase I to further develop the NHBE and other related, and more complex, oral mucosal models. NHBE culture conditions will be optimized and conditions to produce a gingival phenotype and incorporate lamina propria will be determined. Protocols to predict oral irritation will be determined and use of the tissue for transbuccal drug delivery will be studied in a more comprehensive manner. Finally, studies addressing basic oral biology phenomena, namely anti-microbial peptide regulation and HIV infection, will be performed.