GLYCOSYL COMPOSITION OF COLLAGENS

核磁共振分析是在瓦里安Inova 600-MHz光谱仪上进行的。 样品通过冷冻干燥从D2O和 溶解在0.5mLD2O中。1H-核磁共振一维和二维TOCSY(共 相关光谱)，13C-1H-HSQC(异核单量子 关联)，和-HMBC(异核多键关联) 在25℃时获得光谱(测量质子化学位移 相对于内部丙酮标准。一维1H-核磁共振波谱数据 与先前发表的低聚糖的研究结果相当 片段在结构上与本样本建议的片段相同。这个 单糖序列和连接结构通过a 1H-1H同核(TOCSY)和13C-1H-异核系列 相关实验(HSQC、HMBC)。

NMR analysis was performed on a Varian Inova 600-MHz spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL D2O. The 1H-NMR 1-D and 2-D TOCSY (total correlation spectroscopy), 13C-1H-HSQC (heteronuclear single quantum correlation), and -HMBC (heteronuclear multiple-bond correlation) spectra were acquired at 25(C. Proton chemical shifts were measured relative to internal acetone standard. The 1-D 1H-NMR spectral data were comparable to those published previously for an oligosaccharide fragment identical in structure to that proposed for this sample. The monosaccharide sequence and linkage structure were confirmed by a series of 1H-1H-homonuclear (TOCSY) and 13C-1H-heteronuclear correlation experiments (HSQC, HMBC).