Infrastructure for Stem Cell Production & Distribution

描述(由申请人提供)：本申请的目的是将NIH人类胚胎干细胞登记中列出的人类胚胎干细胞系提供给世界各地的研究界。 我们的目标是在第一年能够分配200个等量的人类ES细胞(每个细胞至少含有2x10(6)个细胞)，并在第二年将生产和分配速度翻一番。 我们还将为受助者提供培训，包括：实验室手册、动手培训和网站支持。 为了确保在严格的质量控制程序(良好实验室实践)下扩大高质量的人类胚胎干细胞，我们希望建立一个设备齐全的新实验室，并增加训练有素的生物学家的数量。该团队还将参与旨在改善人类胚胎干细胞的基础研究。探讨人ES细胞系生长需求和分化能力的变化。 这将极大地提高人类胚胎干细胞研究应用的潜力，以及发现潜在的人类胚胎干细胞衍生疗法的速度，以治疗一些最常见和毁灭性的人类疾病。

DESCRIPTION (provided by applicant): The aim of this application is to make the lines of human ES cells listed on the NIH Human ES cells registry available to the research community worldwide. We aim to be able to distribute 200 aliquots of human ES cells (each containing at least 2x10(6) cell) during the first year, and to double the production and distribution rate during the second year. We will also provide training to recipients which will include: lab manual, hands-on training and a web site support. To ensure the scale-up of high quality human ES cells under tight quality control processes (Good Laboratory Practice), we wish to establish a new fully-equipped laboratory and increase the number of the well-trained biologists. The team will also be involved in basic research aiming at improving human ES-cells. And to explore the variation in growth requirements and differentiation capacity of human ES cell lines. This will significantly increase the potential of research applications of human ES cells and the rate of discoveries of potential human ES cell-derived therapies for treating some of the most common and devastating human diseases.