CORE--RESEARCH CYTOGENETICS

研究细胞遗传学和激光捕获显微切割（RCLC）设施提供 核分型、分子细胞遗传学分析和激光捕获显微切割（LCM）。的 1995年7月建立的RCLC设施，由11个国家的所有三个FCCC部门的成员使用。 研究方案。在过去五年中，该机制支助了29名受资助的调查员。在 除了提供全方位的细胞遗传学服务外，RCLC设施还增加了用于分析结构重排的多重荧光原位杂交FISH（M-FISH）和用于分析肿瘤标本和细胞系中DNA拷贝数变化的阵列比较基因组杂交（array-CGH）。现在有两个完整的LCM工作站，包括一个新的AutoPix LCM设备（Arcturus），通过一个完全自动化的系统提供快速和方便的访问纯细胞群。LCM组件由经过培训的病理学家管理。机构总监是人类遗传学项目负责人，细胞遗传学部分由经验丰富且技术熟练的细胞遗传学家管理。2003年，细胞遗传学研究部门进行的检测数量比1999年增加了近四倍，当时该部门还是一个发展中核心设施。1999年，我们对20个样本进行了常规FISH定位和核型分析及CGH分析，而2003年，分析了73个样本，增加了265%。自四年前首次在RCLC设施中引入LCM服务以来，LCM的使用量已从2000年的150小时增加到2003年的570小时，增长了280%。由使用费支持的该基金业务预算的百分比从1999年的11%增加到2003年的20%。虽然对天然基因位点的FISH定位的需求实际上已经结束，但对结构重排或病毒/转基因整合位点的FISH分析，间期FISH以识别非分裂细胞中的基因组改变，以及染色体彩绘和M-FISH以促进复杂或微妙的染色体重排的解释的需求增加了160%。此外，对小鼠模型研究中使用的胚胎干细胞克隆进行核型分析的请求是一项新的频繁需求，平均每年有24项请求。

The Research Cytogenetics and Laser Capture Microdissection (RCLC) Facility provides karyolyping, molecular cytogenetic analyses, and Laser Capture Microdissection (LCM). The RCLC Facility, established in July 1995, is used by Members of all three FCCC Divisions from 11 research Programs. The Facility supported 29 funded investigators over the last five years. In addition to offering a full spectrum of cytogenetic services, the RCLC Facility has added multiplex-fluorescence In situ hybridization FISH (M-FISH) for analysis of structural rearrangements and array-comparative genomic hybridization (array-CGH) for analysis of DNA copy number changes in tumor specimens and cell lines. Two complete LCM workstations are now available, including a new AutoPix LCM apparatus (Arcturus) that provides rapid and convenient access to pure cell populations through a totally automated system. The LCM component is managed by a trained pathologist. The Facility Director is the Program Leader of Human Genetics and the cytogenetics component is managed by an experienced and skilled cytogeneticist. In 2003, the number of tests performed by the Research Cytogenetics component increased nearly four-fold compared to that carried out in 1999, when this was a Developing Core Facility. In 1999, we performed conventional FISH mapping and karyotypic and CGH analyses on 20 samples, whereas in 2003, 73 samples were analyzed, an increase of 265%. From the time when LCM services were first introduced in the RCLC Facility four years ago, LCM usage has increased from 150 hours in 2000 to 570 hours in 2003, an increase of 280%. The percentage of the Facility's operating budget supported by user's fees increased from 11% in 1999 to 20% in 2003. Although demand for FISH mapping of native gene loci has virtually ended, requests increased by 160% for FISH analysis of structural rearrangements or viral/transgene integration sites, interphase FISH to identify genomic alterations in non-dividing cells, and chromosome painting and M-FISH to facilitate interpretation of complex or subtle chromosome rearrangements. In addition, requests for karyotyping of embryonic stem cell clones used in mouse model studies is a new frequent demand averaging 24 requests per year.