ABI PRISM 7900HT SDS 96-Well Sequence Detection System

描述（由申请人提供）：本申请申请用于购买ABI 7900HT序列检测系统的资金，以支持儿科分子生物学核心实验室。该实验室的起源及其持续职能之一是成为儿童健康研究中心的一部分，该中心由儿童健康与发展中心资助。随着Feigin中心专门用于研究的改造，新的分子生物学核心实验室现在不仅满足了CHRC的学者和导师以及儿科部门的其他研究人员的需求；而且对贝勒大学的科学界来说也是如此。该实验室继续作为一个收费单位运作，通过贝勒医学院的拨款与会计办公室为调查人员的账户记账。自2003年将核心实验室迁至费金中心以来，DNA测序和DNA合成业务增长了一倍多。然而，越来越多的人要求核心实验室将实时PCR作为一项服务，这最终导致了这个共享仪器的拨款申请。实时PCR是一种非常强大的技术，目前被研究人员广泛用于各种样品。我们已经确定了18名用户，他们是独立的NIH资助的调查人员。这7个主要用户需要227,000个反应来进行基因表达分析。我们的小用户将需要约24,700个反应进行基因表达分析。根据我们研究人员的预期，在新仪器运行的第一年，有大约251,700个样品的需求。此外，我们认识到用户对SNP分析的需求，这使仪器的应用领域多样化。预计每年将进行27,000次SNP分析。该实验室将在儿科行政机构的支持下运作，由Partha Sen博士管理，并由Morey Haymond博士进行行政监督，Morey Haymond博士是CHRC项目主任，也是贝勒大学儿科部门的副主席。的药。

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an ABI 7900HT Sequence Detection System in support of the Department of Pediatrics Molecular Biology Core Laboratory. The origin of the laboratory and one of its continued functions is to be part of the Children's Health Research Center (CHRC) funded under the auspices of the NICHD. With the remodeling of the Feigin Center exclusively for research, the new Molecular Biology Core Laboratory is now catering to the needs of not only the Scholars and Mentors of the CHRC and other researchers based in the Department of Pediatrics; but also to the Baylor scientific community at large. The laboratory continues to operate as a charge back unit, billing investigators' accounts via the office of Grants & Accounts at Baylor College of Medicine. Business with regards to DNA sequencing and DNA synthesis has increased more than double since the move of the core lab to the Feigin Center in 2003. Nevertheless, there has been an increasing demand on the core lab to include real time PCR as a service, which ultimately resulted in this shared instrument grant application. Real time PCR is a profoundly robust technique that is currently used extensively by the researchers for a variety of samples. We have identified 18 users who are independently NIH funded investigators. The 7 major users have a need of 227,000 reactions for their gene expression analyses. Our minor users will need ~24,700 reactions for gene expression analysis. On the basis of our investigators' anticipation, there is a demand for ~251,700 samples in the first year of operating the new instrument. Additionally, we are cognizant of the need of SNP analysis by the users, which diversifies the field of application of the instrument. A total of 27,000 SNP analyses are anticipated yearly. The laboratory will be operated under the auspices of the administrative structure of the Department of Pediatrics and will be managed by Dr. Partha Sen with administrative oversight from Dr. Morey Haymond, Program Director, CHRC and Vice Chair for Res. for the Dept of Pediatrics, Baylor Coll. of Medicine.