ABI PRISM 7900HT SDS 96-WELL SEQUENCE DETECTION SYSTEM: MOLECULAR BIOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本提案要求为购买ABI 7900 HT序列检测系统提供资金，以支持儿科分子生物学核心实验室。该实验室的起源及其持续的职能之一是成为由NICHD资助的儿童健康研究中心的一部分。随着专门用于研究的Feigin中心的改造，新的分子生物学核心实验室现在不仅满足了CHRC的学者和导师以及儿科系其他研究人员的需求;而且还满足了贝勒科学界的需求。实验室继续作为收费单位运作，通过贝勒医学院的赠款和账户办公室向研究人员的账户收费。自2003年核心实验室搬迁到费金中心以来，DNA测序和DNA合成方面的业务增长了一倍多。然而，对核心实验室的需求越来越大，要求将真实的实时PCR作为一项服务，这最终导致了这一共享仪器赠款申请。真实的时间PCR是一种非常稳健的技术，目前被研究人员广泛用于各种样品。我们已经确定了18名独立的NIH资助的研究人员。7个主要用户的基因表达分析需要227，000个反应。我们的小用户将需要约24，700个反应进行基因表达分析。根据我们调查人员的预期，在新仪器运行的第一年，需要约251，700个样本。此外，我们认识到用户对SNP分析的需求，这使仪器的应用领域多样化。预计每年将进行27，000次SNP分析。该实验室将在儿科系行政结构的主持下运行，由Partha Sen博士管理，并由CHRC项目总监兼贝勒医学院儿科系研究副主席Morey Haymond博士进行行政监督。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an ABI 7900HT Sequence Detection System in support of the Department of Pediatrics Molecular Biology Core Laboratory. The origin of the laboratory and one of its continued functions is to be part of the Children's Health Research Center (CHRC) funded under the auspices of the NICHD. With the remodeling of the Feigin Center exclusively for research, the new Molecular Biology Core Laboratory is now catering to the needs of not only the Scholars and Mentors of the CHRC and other researchers based in the Department of Pediatrics; but also to the Baylor scientific community at large. The laboratory continues to operate as a charge back unit, billing investigators' accounts via the office of Grants & Accounts at Baylor College of Medicine. Business with regards to DNA sequencing and DNA synthesis has increased more than double since the move of the core lab to the Feigin Center in 2003. Nevertheless, there has been an increasing demand on the core lab to include real time PCR as a service, which ultimately resulted in this shared instrument grant application. Real time PCR is a profoundly robust technique that is currently used extensively by the researchers for a variety of samples. We have identified 18 users who are independently NIH funded investigators. The 7 major users have a need of 227,000 reactions for their gene expression analyses. Our minor users will need ~24,700 reactions for gene expression analysis. On the basis of our investigators' anticipation, there is a demand for ~251,700 samples in the first year of operating the new instrument. Additionally, we are cognizant of the need of SNP analysis by the users, which diversifies the field of application of the instrument. A total of 27,000 SNP analyses are anticipated yearly. The laboratory will be operated under the auspices of the administrative structure of the Department of Pediatrics and will be managed by Dr. Partha Sen with administrative oversight from Dr. Morey Haymond, Program Director, CHRC and Vice Chair for Res. for the Dept of Pediatrics, Baylor Coll. of Medicine.