MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。具体目标-在去年的进展报告中提出的对复制复合体的日益了解提出了传统二维（2D） EM成像无法解决的重要问题和机会。所有正链RNA [+)RNA]病毒都与宿主胞内膜结合复制其基因组，而宿主胞内膜重新排列成离散的结构。诺达病毒禽舍病毒（FHV）在感染昆虫细胞时诱导线粒体外膜重排成球形结构。在这些FHV感染的细胞中，RNA复制发生在线粒体外膜。我们的目的是利用电子显微镜断层扫描来更好地了解FHV RNA复制的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aims - The growing understanding of replication complexes presented in last year¿s progress report posed important questions and opportunities that cannot be addressed by conventional two dimensional (2D) EM imaging. All positive-strand RNA [(+)RNA] viruses replicate their genome in association with host intracellular membranes that are rearranged into discreet structures. The Nodavirus Flock House virus (FHV) induces the rearrangement of mitochondrial outer membranes into spherule structures during infection of insect cells. RNA replication occurs at the outer mitochondrial membranes in these FHV infected cells. Our aim is to use electron microscope tomography to better understand the mechanism of FHV RNA replication.